玉米穗行数全基因组关联分析

穗行数是玉米产量的重要组成性状,其遗传解析对高产育种具有指导意义。本文以203份主要玉米自交系为材料,2007年在新疆乌鲁木齐、吉林公主岭和海南三亚进行穗行数测定;采用分布于玉米基因组的41 101个单核苷酸多态性(SNP)标记对穗行数进行关联分析。共鉴定出9个与穗行数显著关联(P0.0001)的SNP,分别位于染色体框1.02、1.10、7.03、8.02、9.06和10.03。8个SNP位于已定位的数量性状座位(QTL)区间内。在显著SNP位点LD区域内发掘出4个候选基因,分别编码含F-box结构域的生长素受体蛋白、玉米kn1蛋白、AP2结构域蛋白和富亮氨酸重复的跨膜蛋白激酶。采用全基因组关联分析策略发掘穗行数基因位点及候选基因,将为克隆控制玉米产量性状基因奠定基础。

Genome-wide Association Analysis of Kernel Row Number in Maize

Kernel row number (KRN) is one of grain yield components in maize (Zea mays L.). Investigation of its genetic architecture will help develop high-yield varieties in maize. In this study, the KRN in a panel of 203 maize inbred lines was detected in Urumqi of Xinjiang, Gongzhuling of Jilin, and Sanya of Hainan in 2007, and used to perform the genome-wide analysis for KRN using MaizeSNP50 BeadChip. A total of nine SNPs were found to be significantly associated with KRN at a threshold of P < 0.0001, which were on chromosome Bins 1.02, 1.10, 7.03, 8.02, 9.06, and 10.03, respectively. Eight of these SNPs were located in the QTL intervals reported previously.Meanwhile, four candidate genes were scanned, encoding auxin signaling F-box containing protein, kn1 protein, AP2 domain containing protein and leucine-rich repeat transmembrane protein kinase respectively. In summary, these identified genes and SNPs will offer essential information for cloning yield-related genes in maize.