| 金花梨基因组DNA提取方法及部位的比较研究 |
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| 引用本文： | 刘遵春,廖明安. 金花梨基因组DNA提取方法及部位的比较研究[J]. 安徽农业科学, 2005, 33(11): 2062-2063 |
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| 作者姓名： | 刘遵春 廖明安 |
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| 作者单位： | 河南科技学院园林学院,河南,新乡,453003;四川农业大学林学园艺学院,四川,雅安,625014 |
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| 摘 要： | 采用CTAB法提取金花梨基因组DNA,在磨样时,1 g鲜样添加0.1 g Vc,提取的DNA产率较高,且极易溶解,能满足RAPO及其他分子生物学研究的需要。试验还发现,最佳提取材料是嫩芽,其次是新梢和成熟的叶片。
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| 关 键 词： | 金花梨 基因组DNA 提取 |
| 文章编号： | 0517-6611（2005）11-2062-02 |
| 收稿时间： | 2005-08-17 |
| 修稿时间： | 2005-08-17 |
Comparison of Genomic DNA Extraction Methods of Jinhua Pear |
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| Liu Zunchunet al. Comparison of Genomic DNA Extraction Methods of Jinhua Pear[J]. Journal of Anhui Agricultural Sciences, 2005, 33(11): 2062-2063 |
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| Authors: | Liu Zunchunet al |
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| Affiliation: | Henan Institute of Science and Technology, Xinxiang, Henan 453003 |
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| Abstract: | Genomic DNA of Jinhua Pear was extracted by CTAB metheds. Results showed that with the dose of 0.1. g/g of Vc added when the samples were triturated, genomic DNA yield was high and it was easiest to dissolve. And it was optimal for RAPD analysis and other molecular biology researches. The results of the experiment also showed that tender bud was the best material, and the next ones were shoot and mature leaf. |
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| Keywords: | Jinhua Pear Genomic DNA Extraction |
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