肉苁蓉中苯乙醇苷化合物的抗氧化活性研究

[目的]对内苁蓉药材中苯乙醇苷化合物的抗氧化活性进行系统分析研究。[方法]采用Photochemiluminescence（PCL）和2,2-diphenyl-1-picrylhydrazyl（DPPH·）2种不同的体系，测量肉苁蓉80％乙醇提取物以及麦角甾苷、异麦角甾苷、松果菊苷的抗氧化活性。[结果]松果菊苷总多酚的含量最高，其次为麦角甾苷、异麦角甾苷和肉苁蓉80％乙醇提取物，分别为753．95、659．94、356．14、14．73mg没食子酸／g。在PCL试验中，肉苁蓉80％乙醇提取物表现出比较强的抗氧化活性，其次为松果菊苷、麦角甾苷和异麦角甾苷；而在DPPH试验中，麦角甾苷表现出较强的抗氧化活性，其次为松果菊苷、异麦角甾苷和肉苁蓉80％乙醇提取物。[结论]该研究可为肉苁蓉药材中苯乙醇苷化合物的药理和生物活性研究提供参考。

Study on the Antioxidant Activities of Phenylethanoid Glycosides from Cistanche deserticola

[Objective]The research aimed to study systematically on the antioxidant activities of phenylethanoid glycosides from Cistanche deserticola .[Method]Two model systems, the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH˙) assay and the photochemiluminescence (PCL) assay, were used to measure the antioxidant activity of the C. deserticola extract, acteoside,isoacteoside and echinacoside.[Result]The results showed that the total phenolic contents (TPCs) in echinacoside, acteoside,isoacteoside and 80% ethanol extract of C. deserticola were 753.95, 659.94, 356.14 and 14.73 mg gallic acid /g sample.The C. deserticola extract showed strong antioxidant activity in PCL experiment, followed by echinacoside, acteoside and isoacteoside. However, echinacoside exhibited strong antioxidant activity in DPPH experiment, followed by acteoside, isoacteoside and the C. deserticola extract.[Conclusion]This research could provide references for the pharmacology and biological activity study of phenylethanoid glycosides from C. deserticola .