| 蝴蝶兰叶外植体发生褐变超微细胞结构和酚类物质分布观察 |
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| 引用本文： | 许传俊,谭茹芳,陈冬茵,赖艳艳,李玲. 蝴蝶兰叶外植体发生褐变超微细胞结构和酚类物质分布观察[J]. 北方园艺, 2010, 0(21): 90-92 |
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| 作者姓名： | 许传俊 谭茹芳 陈冬茵 赖艳艳 李玲 |
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| 作者单位： | 华南师范大学,生命科学学院,广东省植物发育生物工程重点试验室,广东,广州,510631;福建省亚热带植物研究所,福建,厦门,361006;华南师范大学,生命科学学院,广东省植物发育生物工程重点试验室,广东,广州,510631 |
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| 摘 要： | 观察蝴蝶兰叶外植体发生褐变后的细胞亚显微结构发现,细胞膜以及细胞壁扭曲变形甚至断裂,细胞壁降解严重,集聚大量黑色颗粒物质,细胞内出现黑色絮状物质,叶绿体结构破坏。Pauly试剂和亚硝酸钠溶液染色结果表明:褐变外植体存在有较多酚类物质,染色较深。以上结果说明,外植体在褐变过程中,细胞膜完整性被破坏,酚类含量增加促进褐变。
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| 关 键 词： | 蝴蝶兰 外植体 褐变 亚显微结构 酚类 |
Ultrastructure and Distribution of Phenol in Phalaenopsis Browning Leaf Explants |
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| XU Chuan-jun,TAN Ru-fang,CHEN Dong-yin,LAI Yan-yan,LI Ling. Ultrastructure and Distribution of Phenol in Phalaenopsis Browning Leaf Explants[J]. Northern Horticulture, 2010, 0(21): 90-92 |
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| Authors: | XU Chuan-jun TAN Ru-fang CHEN Dong-yin LAI Yan-yan LI Ling |
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| Affiliation: | XU Chuan-jun1,2,TAN Ru-fang1,CHEN Dong-yin1,LAI Yan-yan1,LI Ling1(1.Guangdong Key Lab of Biotechnology for Plant Development,College of Life Science,South China Normal University,Guangzhou,Guangdong 510631,2.Fujian Institute of Subtropical Botany,Xiamen,Fujian 316006) |
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| Abstract: | In the browning Phalaenopsis explants,cell walls were distorted.The cell membranes as well as the cell walls were twist distorts even breakon.And there were abundant of black pellet substance gather in the degraded cell wall.The chloroplasts were shrinked to deep color structure.The results showed that there a lots of phenol in vascular tissues when the browning explant cell treated with Pauly reagent and 10% NaNO2.The integrity of cellular membrane structure of browning explant was destroyed during explant... |
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| Keywords: | Phalaenopsis explant browning ultrastructure phenol |
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