Rapid detection of S-glycoproteins of self-incompatible crucifers using Con-A reaction |
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| Authors: | Takeshi Nishio Kokichi Hinata |
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| Institution: | (1) Faculty of Agriculture, Tohoku University, Sendai, Japan |
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| Abstract: | Summary As S-specific glycoproteins react with concanavalin A (ConA), they could be stained sensitively with ConA-FITC after cellulose acetate isoelectric focusing of stigma proteins in self-incompatible crucifers. By this method the S genotype of an individual plant could be inferred within 4 h using 4 flowers. This simple and rapid method may be applicable for describing S genotypes in practical F1 breeding. |
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| Keywords: | Brassica campestris Brassica oleracea Con-A reaction self-incompatibility S-specific glycoprotein Raphanus sativus |
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