| 玉米分子遗传图谱的SSR和AFLP标记构建 |
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| 引用本文： | 兰进好,张宝石. 玉米分子遗传图谱的SSR和AFLP标记构建[J]. 西北农林科技大学学报(自然科学版), 2004, 32(12): 28-32,37 |
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| 作者姓名： | 兰进好 张宝石 |
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| 作者单位： | 沈阳农业大学,农学院,辽宁,沈阳,110161 |
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| 摘 要： | 以黄早四×Mo17自交形成的191个F2单株为作图群体,利用240对SSR引物和280对AFLP选扩引物,在亲本黄早四和Mo17之间进行了多态性检测,筛选出91对SSR引物和20对AFLP选扩引物用于F2群体分析。利用上述引物组合共检测到248个多态性标记位点,其中的218个标记构建了玉米分子连锁图谱,该图谱覆盖基因组全长2015.5cM,标记间平均间距9.69cM。
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| 关 键 词： | 玉米 遗传图谱 SSR AFLP |
| 文章编号： | 1671-9387(2004)12-0028-05 |
| 收稿时间： | 2004-06-09 |
| 修稿时间： | 2004-06-09 |
Molecular linkage map constructed by SSR and AFLP markers in maize |
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| LAN Jin-hao,ZHANG Bao-shi. Molecular linkage map constructed by SSR and AFLP markers in maize[J]. Journal of Northwest A&F University（Natural Science Edition）, 2004, 32(12): 28-32,37 |
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| Authors: | LAN Jin-hao ZHANG Bao-shi |
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| Affiliation: | (College of Agronomy,Shenyang Agricultural University,Shenyang,Liaoning 110161,China) |
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| Abstract: | 191 F_2 individuals derived from the single cross of inbred lines Huangzao 4 and Mo17 were used as mapping population in maize.240 SSR primer-pairs and 280 selective primer-pairs were used to detect the polymorphisms between parents Huangzao 4 and Mo17.91 SSR primer-pairs and 20 AFLP selective primer-pairs were selected to analyze F_2 population.248 markers were detected by the primer-pairs mentioned above.Only 218 markers were used to construct the molecular genetic linkage map,which span maize genome about 2015.5cM,with average distance of 9.69 cM between markers. |
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| Keywords: | maize linkage map SSR AFLP |
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