中药复方对H9N2亚型禽流感病毒在MDCK细胞增殖的作用

为了筛选对H9N2亚型禽流感病毒（Avian influenza virus，AIV）有独特防治效果的中药复方，本试验基于禽流感发病临床症状、结合中药复方用药基本原则，组方芩根提取液（Qingen extract，QG），以奥司他韦（达菲，DF）为阳性对照药，通过研究药液对神经氨酸酶（neuraminidase，NA）的抑制作用，采用CCK-8法测定药液对犬肾上皮细胞（MDCK）毒性作用，并从病毒吸附、增殖和灭活3个方面做了药效评价。结果表明，QG对NA的抑制作用在一定浓度范围内呈浓度依赖性增高，半数抑制浓度（IC₅₀）约为1 130 μg/mL；QG和DF的最大安全浓度分别为2 500和500 μg/mL，H9N2 AIV的TCID₅₀为10^-4.36/100 μL；QG浓度为156.25~2 500 μg/mL范围内，对H9N2 AIV的病毒毒力有一定抑制作用，并在MDCK细胞上呈现出一定的抗吸附、抑制病毒复制作用。表明QG在对抗H9N2 AIV的NA活性、抑制H9N2 AIV吸附靶细胞和在细胞内复制有一定的作用。

Effect of Chinese Herbal Prescription on the Proliferation of H9N2 in MDCK Cells

To screen traditional Chinese medicines to prevent and treat viral disease H9N2 Avian influenza virus(AIV),Qingen extract(QG)was used to evaluate its anti-H9N2 efficiency through the neuraminidase(NA)inhibition and CCK-8 method from the aspects of virus adsorption,proliferation and inactivation,with oseltamivir(Tamiflu,DF)as positive drug control,based on AIV clinical symptoms and basic principles of traditional Chinese medicine.The results showed that the inhibitory effect of QG on NA increased in concentration dependent manner,and IC 50 was about 1130μg/mL.The maximum safety concentrations of QG and DF were 2500μg/mL and 500μg/mL respectively,and the TCID 50 of H9N2 AIV was 10-4.36/100μL.Among the concentration range of 156.25-2500μg/mL,QG weaken the virulence of H9N2 AIV to a certain extent,and showed anti-attachment and anti-viral replication effects on MDCK cells.In conclusion,QG could inhibit the NA activity of H9N2 AIV,inhibit H9N2 AIV’s attachment and replication to target cells.