铁蛋白1基因在边缘革蜱各发育龄期、吸血期及不同器官中的表达分析

试验旨在了解铁蛋白1（ferritin 1，Fer1）基因在边缘革蜱各发育龄期、吸血期及不同器官中的表达情况。用实时定量荧光PCR法分析了Fer1基因在边缘革蜱各发育阶段、吸血期和不同器官中的相对表达情况，以饥饿雌蜱为基准（1 FC），用2^-△△Ct法计算结果。结果显示，雌性蜱在开始吸血后第72 h Fer1基因的相对表达量最高，在刚刚孵化的饥饿成蜱（雌、雄）、卵及幼蜱中相对表达量较低；在若蜱中相对表达量较高，其中，幼蜱和若蜱在饥饿状态下比同时期饱血蜱的相对表达量高。在雌蜱的不同器官中，以饱血蜱唾液腺为基准（1 FC），半饱血蜱中肠的表达量最高，在卵巢和马氏管不表达；在饱血蜱唾液腺、中肠、卵巢和马氏管中均表达，其中，在卵巢和马氏管中的表达量较高，中肠次之，唾液腺中的表达较低。试验结果表明，边缘革蜱体内Fer1基因的相对表达量随着蜱吸血过程变化而变化，这与蜱体内消化细胞的完善及消化酶在该过程中的变化相关，可为该类蜱铁代谢通路中关键功能基因的研究提供参考。

Expression Profile of Ferritin 1 Gene in Different Developmental Stages,Blood Vampire Period and Different Organs of Dermacentor marginatus

The purpose of this study was to investigate the expression of ferritin 1 (Fer1) gene in different developmental stages,blood sucking stages and different organs of Dermacentor marginatus.Real-time quantitative PCR was used to analyze the expression profile of Fer1 gene in different developmental stages,blood vampire period and different organs of Dermacentor marginatus.The relative expression was calculated using 2^-△△Ct method using starved female ticks as a reference (1 fold-change,1 FC).The results showed that the female adults exhibited highest expression level of Fer1 gene at 72 h after blood feeding.In different development age,newly molted adult ticks (male and female),eggs,and larvae exhibited relatively low expression,while the relative expression level in nymph was higher.The relative expression level in larvea and nymphs under starvation state was higher than that in engorged state.In different tick organs,the expression level of Fer1 gene in midgut if semi-engorged tick was the highest.But there were no expressions detected in ovary and malpighian tube of semi-engorged female tick,and expressions of Fer1 were detected in salivary gland,midgut,ovary and malpighian tube in engorged female ticks,in which ovary and malpighian tube ranking ahead,while salivary gland was the last.The results showed that the relative expression level of Fer1 gene in the tick changed when feeding proceeded which was related to digestive enzymes in the tick,which laid a foundation for the study of biological characteristics and functional genes of this tick.This experiment provided basic data and reference for the study of key functional genes in the metabolic pathway of this tick species.