运输对伊犁马肌肉损伤和氧化应激的影响

试验旨在研究运输对伊犁马肌肉损伤和氧化应激的影响。选取体重相近的5匹24月龄的伊犁母马，环境适应7 d后开展运输试验，其运输过程中禁食禁水，运输总路程约为400 km，运输时间为8 h。分别于运输0（T1）、4（T2）、8（T3）和卸车后12 h （T4）采集马颈静脉血，分离血清，进行相关生理生化指标的测定。结果显示，与T1相比，肌肉损伤生化标志物肌红蛋白（MYO）和肌钙蛋白（cTnT）在T2时刻显著上升（P<0.05），T3和T4时刻极显著上升（P<0.01）；乳酸脱氢酶（LDH）在T3时刻显著上升（P<0.05）；炎症反应生化标志物C反应蛋白（CRP）和白细胞介素-6（IL-6）在T2、T3和T4时刻极显著上升（P<0.01）；皮质醇（COR）在T2和T3时刻极显著上升（P<0.01），在T4时刻显著上升（P<0.05）；总抗氧化能力（T-AOC）、谷胱甘肽过氧化物酶（GSH-Px）和超氧化物歧化酶（SOD）在T2、T3和T4时刻极显著下降（P<0.01）；过氧化氢酶（CAT）在T3和T4时刻显著下降（P<0.05）；活性氧（ROS）在T2和T4时刻显著上升（P<0.05），在T3时刻极显著上升（P<0.01）；丙二醛（MDA）在T3和T4时刻极显著上升（P<0.01）。因此，运输8 h导致了伊犁马肌肉损伤和氧化应激反应的发生，其不良影响在卸车后12 h内不能消除。

Effects of Transportation on Muscle Damage and Oxidative Stress of Ili Horses

The purpose of this study was to investigate the effects of transportation on muscle injury and oxidative stress in Ili horses.Five 24-month old Ili mares with similar body weight were selected.The transportation test was carried out after 7 days of environmental adaptation,no food or water was allowed during transportation.The total transportation distance was about 400 km and the transportation time was 8 hours.The blood samples were collected from horse jugular vein at 0 (T1),4 (T2),8 (T3) of transportation and 12 h (T4) after unloading.The serum samples were determined for related physiological and chemical indicators.The results showed that compared with T1,the muscle damage biochemical markers myoglobin (MYO) and cardiac troponin T (cTnT) increased significantly at T2(P<0.05),and increased extremely sinificantly at T3 and T4 (P<0.01).Lactic dehydrogenase(LDH) increased significantly at T3(P<0.05);The biochemical markers of inflammation C-reactive protein (CRP),interleukin-6 (IL-6) increased extremely significantly at T2,T3 and T4 (P<0.01).Cortisol(COR) increased extremely significantly at T2 and T3 (P<0.01),and increased significantly at T4 (P<0.05).Total antioxidant capacity (T-AOC),glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) decreased extremely significantly at T2,T3 and T4 (P<0.01).Catalase (CAT) decreased significantly at T3 and T4 (P<0.05).Reactive oxygen species (ROS) increased significantly at T2 and T4 (P<0.05),and increased extremely significantly at T3 (P<0.01).Malondialdehyde (MDA) increased significantly at T3 and T4 (P<0.01).Therefore,transportation could cause muscle damage and oxidative stress in Ili horses,and the adverse effects could not be eliminated within 12 h.