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| High-efficiency somatic embryogenesis and morphohistology and histochemistry of somatic embryo development in Larix leptolepis Gordon | |
| 引用本文： | Wang Xiao-xiong Lu Long-dou Hao Huai-qing Teng Nian-jun Chen Tong Guo Yi-ming Yang Ying-gen Guo Zhong-chen Lin Jin-xing. High-efficiency somatic embryogenesis and morphohistology and histochemistry of somatic embryo development in Larix leptolepis Gordon[J]. 中国林学(英文版), 2007, 9(3): 182-188. DOI: 10.1007/s11632-007-0029-8 |
| 作者姓名： | Wang Xiao-xiong Lu Long-dou Hao Huai-qing Teng Nian-jun Chen Tong Guo Yi-ming Yang Ying-gen Guo Zhong-chen Lin Jin-xing |
| 作者单位： | Wang Xiao-xiong1，2 Lu Long-dou2 Hao Huai-qing1 Teng Nian-jun1 Chen Tong1 G uo Yi-ming1 Yang Ying-gen1 Guo Zhong-chen1 Lin Jin-xing1? 1Key Laboratory of Photosynthesis and Molecular Environmental Physiology，Institute of Botany，the Chinese Academy of Sciences，Beijing 100093，P. R. China 2College of Life Sciences，Henan Normal University，Xinxiang 453007，P. R. China |
| 基金项目： | 国家自然科学基金;国家自然科学基金 |
| 摘 要： | A high-efficiency somatic embryogenesis protocol of Japanese larch (Larix leptolepis Gordon) has been established in our investigation. Calli were induced from immature zygotic embryos of female cones of L. leptolepis and then subcultured regularly on to a modified Gupta and Durzan (DCR) basal medium for 5 years. Embryogenic tissues showed distinct morphological changes dur-ing somatic embryo development when they were transferred to a maturation medium supplemented with abscisic acid (ABA) com-pared with the morphology in a medium lacking ABA. Histological observations indicated that polyembryony was a characteristic feature during early embryogeny and somatic embryos at later stages showed normal histodifferentiation. In addition, histochemical analysis revealed that abundant starch granules and proteins accumulated in mature embryos, indicating that they played important roles in the development and regeneration of normal plantlets from somatic embryos on hormone-free germination media |
| 关 键 词： | 脱落酸 组织化学 形体组织学 多胚 高效率肉体胚胎形成 体细胞胚 日本落叶松 |
| 收稿时间： | 2007-04-01 |
| 修稿时间： | 2007-04-01 |
High-efficiency somatic embryogenesis and morphohistology and histochemistry of somatic embryo development in |
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| Wang Xiao-xiong,Lu Long-dou,Hao Huai-qing,Teng Nian-jun,Chen Tong,Guo Yi-ming,Yang Ying-gen,Guo Zhong-chen,Lin Jin-xing. High-efficiency somatic embryogenesis and morphohistology and histochemistry of somatic embryo development in |
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| Authors: | Wang Xiao-xiong Lu Long-dou Hao Huai-qing Teng Nian-jun Chen Tong Guo Yi-ming Yang Ying-gen Guo Zhong-chen Lin Jin-xing |
| Affiliation: | 1. Key Laboratory of Photosynthesis and Molecular Environmental Physiology, Institute of Botany, the Chinese Academy of Sciences,Beijing 100093, P. R. China;College of Life Sciences, Henan Normal University, Xinxiang 453007, P. R. China 2. College of Life Sciences, Henan Normal University, Xinxiang 453007, P. R. China 3. Key Laboratory of Photosynthesis and Molecular Environmental Physiology, Institute of Botany, the Chinese Academy of Sciences,Beijing 100093, P. R. China |
| Abstract: | A high-efficiency somatic embryogenesis protocol of Japanese larch (Larix leptolepis Gordon) has been established in our investigation. Calli were induced from immature zygotic embryos of female cones of L. leptolepis and then subcultured regularly on to a modified Gupta and Durzan (DCR) basal medium for 5 years. Embryogenic tissues showed distinct morphological changes dur-ing somatic embryo development when they were transferred to a maturation medium supplemented with abscisic acid (ABA) com-pared with the morphology in a medium lacking ABA. Histological observations indicated that polyembryony was a characteristic feature during early embryogeny and somatic embryos at later stages showed normal histodifferentiation. In addition, histochemical analysis revealed that abundant starch granules and proteins accumulated in mature embryos, indicating that they played important roles in the development and regeneration of normal plantlets from somatic embryos on hormone-free germination media |
| Keywords: | Larix leptolepis Gordon abscisic acid (ABA) histochemistry morphohistology polyembryony |
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