绿茶儿茶素类生物标记物的检测及应用

绿茶被认为具有多种生理功效,但临床实验和流行病学研究仍存有争议。绿茶儿茶素类生物标记物检测方法的建立是为了从代谢物水平研究绿茶及其提取物对人体的生理效用,有助于阐释绿茶生理功效的作用机理,并避免或减轻由于摄入样品化学组成差异和生物个体差异对实验结果的影响。儿茶素类化合物EGCG、EGC、EC和ECG及其派生物是常用的绿茶儿茶素类生物标记物,主要检测方法有高效液相色谱-电化学检测法、液相色谱-串联质谱法等。本文综述了绿茶儿茶素类生物标记物的种类、备样方法和检测手段,分析、讨论了基于绿茶儿茶素类生物标记物开展的绿茶生物利用率研究,以及临床实验和流行病学研究结果,提出在后续研究中应进一步完善和规范人体中儿茶素类生物标记物的备样和检测方法,并可尝试寻找在生物体内比较稳定的、更能反映绿茶及其提取物摄入水平的新型生物标记物。

Determination of Green Tea Catechin Biomarkers and It′s Relative Application

Green tea has been considered to have many health benefits, but there is still controversy about the clinical and epidemiological results. Development of green tea catechin biomarkers was to evaluate the physiological effects of green tea from a metabolic point of view, which will be helpful to interpret the functional mechanism of green tea in vivo and also avoid or reduce the influences of chemical compositions of samples intake or individual differences in human studies. Catechin compounds EGCG, EGC, EC, C, ECG and their derivatives are common green tea catechin biomarkers that have many determination methods including HPLC-EC, LC-MS and so on. This paper reviewed the types of green tea catechin biomarkers and their preparation and determination methods, discussed the results of biomarker-level based bioavailability studies, clinical trials and epidemiological studies, proposed suggestions for future research, such as further improvement and standardization of the preparation and determination methods of catechin biomarkers as well as development of new green tea biomarkers with higher stability in human body and sensitivity in reflecting their intake levels.