| 一个新的柽柳类萌芽素基因ThGLP的结构及其表达分析 |
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| 引用本文： | 李慧玉,姜静,王珊.一个新的柽柳类萌芽素基因ThGLP的结构及其表达分析[J].林业研究,2010,21(3):323-330. |
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| 作者姓名： | 李慧玉 姜静 王珊 |
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| 作者单位： | 东北林业大学林木遗传育种与生物技术教育部重点实验室,哈尔滨150400 |
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| 基金项目： | national natural science foundation，Central university basic scientific business specific foundation，Genetically modified organisms breeding major projects |
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| 摘 要： | 萌芽素和类萌芽素蛋白在不同植物的各个生长阶段和胁迫相关过程中起到不同的作用。本研究首次从刚毛柽柳cDNA文库中获得类萌芽素蛋白全长基因ThGLP,该基因编码225个氨基酸,含有植物萌芽素和类萌芽素蛋白的功能序列。通过进化树分析发现该基因屑于真正萌芽素亚家族。利用实时定量PCR方法研究了该基因在PEG、NaCl、低温、CdCl2和ABA胁迫下不同时间的表达模式。结果显示PEG、NaCl、低温、CdCl2和ABA处理均能诱导ThGLP基因在柽柳的根和叶中的表达。结果表明ThGLP在柽柳根和叶中表达,参与非生物胁迫应答并由ABA依赖的信号传导途径调控。
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| 关 键 词： | 类萌芽素蛋白 刚毛柽柳 非生物胁迫 基因表达 |
Expression analysis of <Emphasis Type="Italic">ThGLP</Emphasis>, a new germin-like protein gene,in <Emphasis Type="Italic">Tamarix hispida</Emphasis> |
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| Hui-yu?Li,Jing?Jiang,Shan?Wang,Fei-fei?Liu.Expression analysis of ThGLP, a new germin-like protein gene,in Tamarix hispida[J].Journal of Forestry Research,2010,21(3):323-330. |
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| Authors: | Hui-yu Li Jing Jiang Shan Wang Fei-fei Liu |
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| Institution: | (1) Department of Plant Pathology, The University of Georgia, Tifton, GA 31793, USA;(2) Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China;(3) Plant Genetic Resources Conservation Unit, USDA-ARS, Griffin, GA, USA;(4) Crop Genetics and Breeding Research Unit, USDA-ARS, Tifton, GA, USA;(5) Crop Protection and Management Research Unit, US Department of Agriculture, Agricultural Research Service, Tifton, GA 31793, USA; |
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| Abstract: | Germin and Germin-like protein (GLP) have various proposed roles in plant developmental stages and stress-related processes. A novel GLP cDNA clone was isolated from a cDNA library of Tamarix hispida.ThGLP, coded 225aa, possesses conserved motif of plant germin and Germin-like protein. ThGLP belongs to true germin subfamily through phylogenetic analyses. Gene expression profiles in roots and leaves were evaluated using real-time quantitative RT-PCR. The results show that the gene was highly induced by drought, salt, low temperature, CdCl2 and abscisic acid treatments. Our results demonstrate that the ThGLP gene is expressed in leaves and roots, is involved in different abiotic stress responses and controlled by an ABA-dependent signaling pathway. |
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| Keywords: | germin-like protein (GLP) Tamarix hispida abiotic stress gene expression |
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