| 鸭茅高效丛生芽体系的建立及耐盐植株的再生 |
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| 引用本文： | 晁相蓉,宋玲玲,胡孝瑞,姜素云,杨爱芳.鸭茅高效丛生芽体系的建立及耐盐植株的再生[J].山东农业科学,2005(1):7-10. |
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| 作者姓名： | 晁相蓉 宋玲玲 胡孝瑞 姜素云 杨爱芳 |
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| 作者单位： | 1. 山东省卫生学校,山东,济南,250002
2. 山东大学生命科学学院,山东,济南,250100 |
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| 基金项目： | 国家植物转基因专项(J2002-B-006)，山东省优秀中青年科学家科研奖励基金(2004BS06004)资助。 |
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| 摘 要： | 取鸭茅无菌黄化苗生长点处的2～3mm长切段为材料,在附加不同浓度6-BA和2,4-D组合的培养基上诱导丛生芽发生。丛生芽块在继代培养中迅速增生。通过在培养基中加盐筛选,获得了耐受1 5%NaCl的耐盐小芽。这些小芽在无NaCl的培养基上恢复培养3代后转回附加1 5%NaCl的培养基上继续培养,表现出比对照小芽明显提高的耐盐性和增殖率。耐盐小芽在生根培养基上生根后移栽大田,成活率达90%以上,获得了耐盐性提高的鸭茅新种质。
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| 关 键 词： | 鸭茅 离体培养 丛生芽 耐盐植株再生 |
| 文章编号： | 1001-4942(2005)01-0007-04 |
High efficiency induction of multiple shoots from orchardgrass (Dactylis glomerata L. ) and regeneration of salt - tolerant plantlets |
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| CHAO Xiang-rong,SONG Ling-ling,HU Xiao-rui,JIANG Su-yun,YANG Ai-fang.High efficiency induction of multiple shoots from orchardgrass (Dactylis glomerata L. ) and regeneration of salt - tolerant plantlets[J].Shandong Agricultural Sciences,2005(1):7-10. |
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| Authors: | CHAO Xiang-rong SONG Ling-ling HU Xiao-rui JIANG Su-yun YANG Ai-fang |
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| Institution: | CHAO Xiang-rong~1,SONG Ling-ling~2,HU Xiao-rui~2,JIANG Su-yun~2,YANG Ai-fang~ |
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| Abstract: | Multiple shoots were induced from the 2～3 mm segments of the seedling tips of aseptically orchardgrass and proliferated vigorously on media supplemented with different combinations of 2,4-D and 6-BA. Proliferating shoots were subcultured on media containing 1.5% NaCl for 3 continuous generations and salt-tolerant shoots were obtained. Salt-tolerant shoots, after being subcultured for 3 generations on NaCl-free medium, showed higher salt-tolerance and proliferating rate than the control on media supplemented with 1.5% NaCl. Salt-tolerant plantlets with vigorous root system were transplanted to pot and the surviving rate was more than 90%. |
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| Keywords: | Dactylis glomerata L In vitro culture Multiple bud clumps Salt-tolerant plantlet regeneration |
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