响应面法优化森吉木霉M75菌株发酵培养条件

目的 利用响应面法优化森吉木霉M75菌株的液体发酵培养条件,使其无菌滤液拮抗松枯梢病病原菌松球壳孢菌的抑菌活性得到有效提高,获得森吉木霉M75高效抑菌的发酵培养基配方。 方法 采用Plackett-Burman试验设计、最陡爬坡试验,结合响应面设计法优化森吉木霉M75的发酵培养基,采用Design Expert 11.0软件对试验数据进行处理分析。 结果 得到了培养基配方中对森吉木霉M75发酵滤液抑菌活性影响最显著的3个主要因素：葡萄糖、温度和转速,同时得到了最佳发酵培养浓度为：葡萄糖2.75%,胰蛋白胨1.2%,硫酸锌0.2%,马铃薯浸汁1 000 mL,温度31.0 ℃、转速195 r·min−1,接种量5%,装液量180 mL,发酵培养5 d。 结论 获得了在实验室条件下森吉木霉M75菌株高效抑菌的发酵培养基配方和培养条件,为森吉木霉菌株后续的研究提供了有效依据。

Optimization of Fermentation Conditions of Trichoderma songyi M75 Strain by Response Surface Methodology

Objecive To improve the antibacterial activity of its sterile filtrate against the pathogen of pine shoot blight and obtain the high-efficiency antibacterial fermentation medium formula of Trichoderma songyi M75, response surface method was used to optimize the liquid fermentation culture conditions of T. songyi M75 . Method Plackett-Burman experiment, the steepest ascent method, and response surface method were used to optimize the fermentation medium of T. songyi M75, and the experiment data were analyzed using Design Expert 11.0. Result The three main influential factors were glucose, temperature and speed and the optimal fermentation culture concentrations conditions were: glucose 2.75%, tryptone 1.2%, zinc sulfate 0.2%, potato juice 1000 mL, temperature 31.0 ℃, rotation speed 195 r·min−1, inoculation volume 5%, liquid volume 180 mL, fermentation culture 5 d. Conclusion This study greatly improves the antibacterial activity and obtains the optimal culture conditions, which provides an effective basis for the follow-up study of T. songyi strains.