绿汁发酵液和纤维素酶对香蕉叶青贮品质的影响

为开发利用香蕉叶生产青贮饲料，研究绿汁发酵液和纤维素酶对香蕉叶青贮品质的影响，在香蕉叶青贮中以香蕉叶单独青贮为对照组，设置如下处理：添加2mL·kg-1绿汁发酵液（FGJ）组；分别添加0.05、0.15、0.25g·kg-1纤维素酶（CEL1、CEL2和CEL3）组；分别添加2mL·kg-1FGJ＋0.05g·kg-1CEL、2mL·kg-1FGJ＋0.15g·kg-1CEL和2mL·kg-1FGJ＋0.25g·kg-1CEL混合（MIX1、MIX2和MIX3）组。每个处理重复3次。常温下贮存60d开封，测定青贮的发酵品质和化学成分。结果表明，①较对照组，处理组的pH、气体损失率（GLR）极显著或显著降低（P＜0.01或P＜0.05）。②较FGJ组，3个MIX组的干物质回收率（DMR）极显著升高（P＜0.01）；较CEL组，同纤维素酶水平的MIX组的DMR、可溶性碳水化合物（WSC）极显著增加（P＜0.01），氨态氮/总氮（AN/TN）极显著降低（P＜0.01）；③在3个MIX组中，随纤维素酶添加水平升高，pH呈下降趋势，DMR、WSC呈递增趋势。结果表明，绿汁发酵液和纤维素酶都能显著改善香蕉叶青贮的品质，二者混合添加的效果优于单独添加，2mL·kg-1FGJ＋0.25g·kg-1CEL混合处理的青贮品质最优。

Effects of Fermented Green Juice and Cellulase on the Fermentation Quality of Banana Leave Silage

To research the effect of fermented green juice and cellulase on the fermentation quality of banana leaves silage ,banana leaves single silage was set as the control group and other silages were set up into the following groups with the different additives ：2 mL · kg -1 fermented green juice （FGJ） group ,0.05 ,0.15 and 0.25 g · kg -1 of cellulose groups （CEL1 ,CEL2 and CEL3） and 2 mL · kg -1 FGJ＋0.05 g · kg -1 cell,2 mL · kg -1 FGJ ＋0.15 g · kg -1 celland 2 mL · kg -1 FGJ＋ 0.25 g · kg -1 cellmixed groups （MIX1 ,MIX2 and MIX3） respectively . Then ,they were ensiled at room temperature and opened 60 days later .In the end ,the fermentation quality and the chemical composition were analyzed .The same results were obtained in all three replicates .Our results showed that the pH and GLR of the other groups are decreased （P＆lt; 0.01 or P＆lt; 0.05） ,compared with the control group . Meanwhile ,the DMR of three MIX groups were increased （P＆lt;0.01） compared with the FGJ ,and the DMR and WSC of MIX groups were increased （P＆lt;0.01） ,but the AN/TN were reduced （P＆lt;0.01） ,compared with the cellgroups with the same level′s cellulase enzyme .Furthermore ,the pH was declining on trend ,DMR and WSC were increased with the levels of cellulose in three MIX groups .Our results suggested that fermented green juice and cellulase improved the quality of banana leaves silage ,and the effect of M IX3 is the best .