| 酒红球盖菇液体摇瓶培养基优化 |
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| 引用本文： | 聂建军,李彩萍,杨玉画,李兆斌. 酒红球盖菇液体摇瓶培养基优化[J]. 山西农业科学, 2011, 39(10): 1073-1075. DOI: 10.3969/j.issn.1002-2481.2011.10.13 |
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| 作者姓名： | 聂建军 李彩萍 杨玉画 李兆斌 |
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| 作者单位： | 1. 山西省农业科学院农业资源与经济研究所,山西太原,030006
2. 山西省农业科学院农业环境与资源研究所,山西太原,030006 |
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| 基金项目： | 山西省科技成果推广项目(2006071021) |
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| 摘 要： | 通过酒红球盖菇菌丝在液体摇瓶培养基内培养,结果表明,液体培养最佳碳源是蔗糖、最佳氮源是麸皮;以菌丝体生物量为测量指标,采用正交试验方法筛选出酒红球盖菇液体摇瓶最佳培养基为:蔗糖2%,可溶性淀粉1%,麸皮2%,蛋白胨0.5%,KH2PO4 0.1%,MgSO4 0.05%。
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| 关 键 词： | 酒红球盖菇 液体 摇瓶 培养基 优化 |
Optimization of the Liquid Medium in Shaking Flask of Stropharia rugoso-annulate |
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| NIE Jian-jun,LI Cai-ping,YANG Yu-hua,LI Zhao-bin. Optimization of the Liquid Medium in Shaking Flask of Stropharia rugoso-annulate[J]. Journal of Shanxi Agricultural Sciences, 2011, 39(10): 1073-1075. DOI: 10.3969/j.issn.1002-2481.2011.10.13 |
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| Authors: | NIE Jian-jun LI Cai-ping YANG Yu-hua LI Zhao-bin |
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| Affiliation: | NIE Jian-jun1,LI Cai-ping2,YANG Yu-hua1,LI Zhao-bin1(1.Institute of Agricultural Resources & Economics,Shanxi Academy of Agricultural Sciences,Taiyuan 030006,China,2.Institute of Agricultural Environment & Resources,China) |
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| Abstract: | The results of Stropharia rugoso-annulate mycelium culture in liquid medium in the shaking flask,showed that the best carbon source for the liquid medium was sucrose and the best nitrogen source bran;Using mycelium as measurement indicators on biomass and the orthogonal experiment methods,the best liquid medium in shaking flask of Stropharia rugoso-annulate was selected as: sucrose 2%,soluble starch 1%,bran 2%,peptone 0.5%,KH2PO4 0.1%,MgSO4 0.05%. |
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| Keywords: | Stropharia rugoso-annulate liquid shaking flask medium optimization |
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