| 抗草丁膦转基因玉米不同DNA提取方法及PCR检测 |
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| 引用本文： | 杨慧珍,车丽,任志强,肖建红. 抗草丁膦转基因玉米不同DNA提取方法及PCR检测[J]. 山西农业科学, 2011, 39(9): 925-927,1028. DOI: 10.3969/j.issn.1002-2481.2011.09.01 |
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| 作者姓名： | 杨慧珍 车丽 任志强 肖建红 |
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| 作者单位： | 1. 山西省农业科学院作物科学研究所,山西太原,030032
2. 山西省农业科学院现代农业研究中心,山西太原,030031 |
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| 基金项目： | 国家转基因生物新品种培育重大专项(2009ZX08003-005B); 山西省农业科学院“十二五”育种工程项目(11yzgc150) |
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| 摘 要： | 以改良PEX,CTAB和SDS方法从转基因玉米材料中提取基因组DNA,并以外源CaMV35S启动子、bar基因及内源基因Zein的序列设计引物,用PCR方法扩增预期大小的DNA片段。结果显示,3种方法提取的DNA用以PCR检测都获得了预期大小的扩增片段,但改良PEX提取方法更省时省力。研究认为,改良PEX提取方法是一种简捷、快速和高效的植物DNA提取方法。
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| 关 键 词： | 玉米 转基因 草丁膦 PCR检测 DNA提取方法 |
Study on DNA Isolation Methods and PCR Detection of Transgenetic Maize Resistant to Glufosinae |
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| YANG Hui-zhen,CHE Li,REN Zhi-qiang,XIAO Jian-hong. Study on DNA Isolation Methods and PCR Detection of Transgenetic Maize Resistant to Glufosinae[J]. Journal of Shanxi Agricultural Sciences, 2011, 39(9): 925-927,1028. DOI: 10.3969/j.issn.1002-2481.2011.09.01 |
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| Authors: | YANG Hui-zhen CHE Li REN Zhi-qiang XIAO Jian-hong |
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| Affiliation: | YANG Hui-zhen1,CHE Li1,REN Zhi-qiang2,XIAO Jian-hong2(1.Institute of Crop Sciences,Shanxi Academy of Agricultural Sciences,Taiyuan 030032,China,2.Research Centre of Modern Agriculture,Taiyuan 030031,China) |
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| Abstract: | Using maize genomic DNA isolated from transgenic maize plants by PEX,CTAB and SDS methods,as a temples for PCR amplification,the primers were designed according to the sequences of CaMV35S promoter of exogenous gene,bar gene and endogenous gene Zein.The results of PCR amplification showed that 3 expected length fragments were amplificated from all genomic DNA that isolated by the above methods,however,the PEX method was less time and labor consuming.It is concluded that PEX method is a simple,fast and effic... |
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| Keywords: | maize transgenetic glufosinate PCR detection extraction methods for DNA |
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