Isolated pea anther culture |
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| Authors: | S V Bobkov |
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| Institution: | (1) Max F. Perutz Laboratories, University Departments at the Vienna Biocenter, Department of Plant Molecular Biology, Vienna University, A-1030, Dr. Bohrgasse 9, Vienna, Austria;(2) Department of Tissue Culture and Gene Transformation, Agricultural Biotechnology Research Institute of Iran, Mahdasht Road, P.O. Box 31535-1897, Karaj, Iran; |
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| Abstract: | Callus tissues and green globular embryoids are obtained in a pea anther culture on a medium with 2,4-D after cold (4°C) treatment
of buds. In experiments with the use of treating buds with cold (4°C) and anther culture with heat (32 and 35°C), green embryogenic
and morphogenetic calli are induced and regenerant plants are obtained. The origin of the regenerants is established by genetic
markers. |
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