| 鸡肠道抗菌肽的提取及活性研究 |
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| 引用本文： | 谭淑樱,王海英,祁克宗,彭开松. 鸡肠道抗菌肽的提取及活性研究[J]. 水禽世界, 2009, 0(7): 3-6 |
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| 作者姓名： | 谭淑樱 王海英 祁克宗 彭开松 |
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| 作者单位： | 安徽农业大学动物科技学院,合肥,230036 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，安徽省优秀青年基金 |
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| 摘 要： | 取新鲜鸡肠黏膜超声波破碎,乙酸浸提,经Sephadex G-50层析后,收集具有抑菌活性的组分进行超滤离心,抑菌实验表明滤过物具有抑菌活性.滤过物经SDS-PAGE分析为一个条带,分子量约为6.078KD,得到电泳纯的鸡肠道抗微生物肽.该肽具有一定的耐热性,但经90℃以上热处理后活性下降较大;pH值对其活性影响较大,在pH6～8时活性最强.
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| 关 键 词： | 鸡 肠黏膜 抗微生物肽 温度 pH值 |
The Preparation and Activity of an Antimicrobial Peptide from Chicken Intestine |
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| TAN Shu-Ying,WANG Hai-Ying,QI Ke-Zong,PENG Kai-Song. The Preparation and Activity of an Antimicrobial Peptide from Chicken Intestine[J]. Poultry Science, 2009, 0(7): 3-6 |
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| Authors: | TAN Shu-Ying WANG Hai-Ying QI Ke-Zong PENG Kai-Song |
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| Affiliation: | College of Animal Science and Veterinary Medicine;Anhui Agricultural University;Hefei 230036 |
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| Abstract: | After the intestinal mucous membranes of chicken were treated by ultrasonic treatment and acid extraction,an antimierobial peptide was isolated from the intestine of chicken by Sephadex G-50 gel filtration chromatography and centrifugal ultrafiltration.The filtration material was analyzed by SDS-PAGE and the result showed that an antimierobial peptide was purified and its molecule weight was about 6.078kD.The antimicrobial peptide was heat-resistant,but its activity descended obviously after heated at 90℃.The activity of the antimierobial peptide was affected by pH and it was the strongest at pH6～8. |
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| Keywords: | chicken intestinal mucous membrane antimicrobial peptide temperature pH value |
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