枳椇不同部位总黄酮含量比较及其总黄酮提取工艺研究

比较了枳椇各部位(叶子、枝干、果梗、果渣、种子、种壳)总黄酮的含量,获得了枳椇叶中总黄酮的最佳提取工艺。分别采用盐酸-镁粉法、三氯化铝法、醋酸铅法对枳椇各部位总黄酮含量进行定性分析,以芦丁为对照品,经Al Cl3显色和分光光度法进行定量分析,并通过正交法确定枳椇叶总黄酮的最佳提取工艺。结果表明,枳椇各部位总黄酮的含量分别为:种子5.081 mg/g、枝干3.452 mg/g、种壳0.899 mg/g、果梗0.871 mg/g、果渣0.835 mg/g,其中枳椇叶中总黄酮含量最高,为6.314 mg/g。经过正交试验分析,索氏提取法提取枳椇叶中总黄酮的最佳工艺参数为:乙醇浓度75%,料液比1∶50(g/m L),提取时间9 h,在该工艺条件下枳椇叶中总黄酮含量为14.8 mg/g。

Comparison of the Total Flavonoids Content from Different Parts of Hovenia acerba and Extration Technology Study

In order to select the best macroporous resin, and investigate their kinetics curve and static adsorption performance, the characteristics of adsorption and separation of four types of macroporous resin including FL-1, FL-2, FL-3, AB-8 were compared on their adsorption and desorption rates for purifing flavonoids from Alpinia galangal (L.) Willd in this paper. The results showed that, FL-3 resin had higher adsorption and desorption capacity, it exhibited best adsorption performance when loading buffer concentration was 0.314 mg/mL, pH value was 4 and the flow velocity was 1 mL/min. And when eluant ethyl alcohol content was 80%, pH value was 5, the flow velocity was 1.5 mL/min, the largest desorption quantity of FL-3 macroporous resin reached 93.2%. Meanwhile, the purity of flavonoids was up to 40.3%. It can be used to purify and separate flavonoids from Alpinia galangal (L.) Willd.