| 杏叶片DNA提取和荧光AFLP反应体系的建立 |
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| 引用本文： | 张俊环,孙浩元,杨丽,王玉柱. 杏叶片DNA提取和荧光AFLP反应体系的建立[J]. 北方园艺, 2010, 0(19) |
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| 作者姓名： | 张俊环 孙浩元 杨丽 王玉柱 |
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| 作者单位： | 北京市农林科学院林业果树研究所; |
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| 基金项目： | 北京市自然科学基金重点资助项目，国家"十一五"科技支撑计划资助项目，国家"十一五"公益性行业科研专项资助项目 |
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| 摘 要： | 以杏嫩叶为材料,利用改进的CTAB法,提取到高质量的杏叶片总DNA,通过优化酶切、连接、预扩增、选择性扩增等试验条件,建立了高效稳定的杏荧光AFLP反应体系;筛选出多态性好的12对引物组合,均获得稳定、清晰的扩增图谱,可进行杏品种鉴定、种质资源的遗传多样性和亲缘关系分析等研究。
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| 关 键 词： | 杏 DNA提取 荧光AFLP标记 |
Extraction of DNA and Establishment of Fluorescent-AFLP Techniques in Leaves of Apricot |
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| ZHANG Jun-huan,SUN Hao-yuan,YANG Li,WANG Yu-zhu. Extraction of DNA and Establishment of Fluorescent-AFLP Techniques in Leaves of Apricot[J]. Northern Horticulture, 2010, 0(19) |
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| Authors: | ZHANG Jun-huan SUN Hao-yuan YANG Li WANG Yu-zhu |
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| Affiliation: | ZHANG Jun-huan,SUN Hao-yuan,YANG Li,WANG Yu-zhu(Institute of Pology and Forestry,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100093) |
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| Abstract: | The high quality DNA required for construction of AFLP analysis system was isolated with an improved method of CTAB from young leaves of apricot.A fluorescent-AFLP reaction system was constructed for genetic analysis of apricot by optimizing DNA restriction-ligase reaction,pre-amplification and selective amplification.The clear and stable amplification patterns could be obtained from 12 pairs primer combinations with good polymorphism,which were selected from 64 pairs AFLP primer combinations.These results ... |
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| Keywords: | apricot extraction of DNA fluorescent-AFLP |
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