辣椒抗疫病性状遗传及其相关AFLP标记分析

以93-100-17-1-0×茄门组合为基础，建立F2代作图群体。从感受性、诱导性和稳定性等3个方面进行遗传分析，发现93-100-17-1-0的抗疫病性状是由多基因或寡基因所控制的。根据AFLP分析，利用Mapmaker/exp (Version 3.0)作图软件构建辣椒的分子连锁图谱，结合winQTLCART(Version 1.15)，对辣椒抗疫病的基因定位，将与感受性有关的QTL位点定位在第5条连锁群上，与诱导性有关的QTL位点分别定位在第1、第2、第5、第8条连锁群，与稳定性有关的QTL位点定位在第1、第2、第5、第7条连锁群，各QTL位点可解释表型变异率在64%以上。

Inheritance of Resistant Traits Related to Phytophthora capsici in Capsicum annuum and Analysis of Relative AFLP Markers

For mapping and characterizing QTLs related to Phytophthora capsici(PC)resistance in pepper(Capsocum annuum),a molecular linkage map was constructed with AFLP markers in an intraspecific F2 population by crossing 93-100-17-1-0,a highly resistant to PC,with Qiemen,an inbred line susceptible to PC.A total of 165 F2 plants from the cross between 93-100-17-1-0 and Qiemen were used for identification of DNA markers linked to PC resistance.The results of the genetic statistical analysis using three different criteria,receptivity,inductivity and stability,indicated that the resistance of 93-100-17-1-0 to PC was controlled by polygenes or oligogenes.A genetic map was constructed using the software Mapmaker/exp,which contained 11 linkage groups with 46 linked molecular markers and covered 1381.5 cM of the whole genome.Using the three different criteria as traits,18 QTLs localizing on LG1,LG2,LG5,LG7 and LG8,which explained over 64% of the phenotypic variance,were identified using the function interval mapping and composite interval mapping of the software winQTLCART.The most significant QTL localized on LG5 from 128 cM to 132 cM explained 68% of the phenotypic variance and whose genetic distance was only 4 cM.