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| 梨ACC氧化酶基因(ACO)的片段克隆及其RNAi载体构建 | |
| 引用本文： | 胡钟东,乔玉山,王三红,姚泉洪,章镇. 梨ACC氧化酶基因(ACO)的片段克隆及其RNAi载体构建[J]. 果树学报, 2006, 23(6): 877-879 |
| 作者姓名： | 胡钟东 乔玉山 王三红 姚泉洪 章镇 |
| 作者单位： | 1. 南京农业大学园艺学院,南京,210095;江西农业科学院园艺研究所,南昌,330200 2. 南京农业大学园艺学院,南京,210095 3. 上海市农业科学院生物技术研究中心,上海,201106 |
| 基金项目： | 江苏省高技术研究发展计划项目 , 南京农业大学校科研和教改项目 |
| 摘 要： | 以若光梨成熟果实为材料,提取总RNA,在ACC氧化酶基因(ACO)cDNA序列同源性较高区域设计一对特异引物,利用RT-PCR克隆了ACC氧化酶(ACO)基因片段。将ACO反义基因、与副球菌中类胡罗卜素合成有关的间隔基因YYT和ACO正义基因3个片段串联在一起,经鉴定后,插入到植物表达载体中,构建成能够表达ACC氧化酶基因的双链RNA的植物载体pYF028,为耐贮砂梨的遗传转化创造条件。 |
| 关 键 词： | 梨 ACC氧化酶(ACO) RT-PCR 克隆 RNAi载体构建 |
| 文章编号： | 1009-9980（2006）06-877-03 |
| 收稿时间： | 2006-05-13 |
| 修稿时间： | 2006-09-15 |
Cloning of ACC oxidase gene (ACO)of pear (Pyrus pyrifolia) and construction of its corresponding RNAi vectors |
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| HU Zhong-dong,QIAO Yu-shan,WANG San-hong,YAO Quan-hong,ZHANG Zhen. Cloning of ACC oxidase gene (ACO)of pear (Pyrus pyrifolia) and construction of its corresponding RNAi vectors[J]. Journal of Fruit Science, 2006, 23(6): 877-879 | |
| Authors: | HU Zhong-dong QIAO Yu-shan WANG San-hong YAO Quan-hong ZHANG Zhen |
| Affiliation: | 1 College of Horticulture, Nanjing Agricultural University, Nanjing , Jiangsu 210095 China; 2Institute of Horticulture ,Jiangxi Academy of Agricultural Sciences, Nanchang. Jiangxi 330200 China; 3Agri-biotechnology Research Center, Shanghai Academy of Agricultural Sciences, Shanghai 201106 China |
| Abstract: | The total RNA was isolated from the ripe fruit of Wakahikari pear cultivar (Pyrus pyrifolia NaKai cv. Wakahikari). A pair of special primers was designed according to the high homology region of ACC oxidase gene. The antisense and sense ACC oxidase (ACO) gene were obtained by RT-PCR and tandem ligated with the gene YYT as internal sequence, which is relative to carotenoid synthesization in subcocci. The fusion gene was inserted into plant vector to construct the recombinant plasmid pYF028, which could express dsRNA of ACC oxidase gene. In such way it provided the possibility of transformation to improve the storage life of pear cultivars. |
| Keywords: | Pear ACC oxidase RT-PCR Clone Construction of RNAi vectors |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |