不同宿主源棘球蚴包囊液抗原的SDS—PAGE分析

本文利用ＳＤＳ－ＰＡＧＥ对不同宿主源棘球蚴囊液抗原的多肽组成进行了分析和比较，旨在为免疫诊断抗原的分离、鉴定和纯化奠定基础，为细粒棘球绦虫种内变异和株的鉴定提供参考指标。结果表明，在还原条件下，绵羊棘球蚴囊液抗原共有多肽带１９条，其中６６和５９ＫＤ多肽带为主带，４０、３４．５、３３、２４．５和１４ＫＤ多肽带次之。牛源囊液抗原共有多肽带１２条，６６和５９ＫＤ多肽带也为主带，２４．５ＫＤ多肽带次之。人源囊液抗原共有多肽带１３条，６６、４０、２０．５和１４ＫＤ多肽带为主带，５９ＫＤ多肽带近于缺乏，分子量在５９和２４ＫＤ之间的带明显偏少，而７１、６９、６８和２０．５ＫＤ多肽带为自身特有。３种不同宿主源棘球蚴囊液抗原其多肽组成均很复杂，羊、牛源囊液抗原的ＳＤＳ－ＰＡＧＥ图谱较相似，而人源与此差异明显。初步认为绵羊和牛源囊液抗原在免疫诊断中具有可替代性。

ANALYSIS OF HYDATID FLUIDS FROM SEVERAL HOST ECHINOCOCCUS GRANULOSUS ISOLATES WITH SDS PAGE

A comparison of Peptide composition was made,by sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS PAGE),of crude hydatid fluids from ovine ,bovine and human E.granulosus isolates.Under reducing conditions,Ovine samples showed 19 peptide bands,among them 66KD and 59KD were major ones,and 40,34.5,33,24.5KD secondly.Bovine samples had 12 peptide bands,of which 66KD and 59KD were also major ones,and 24.5KD was in the second place.Human hydatid fluid yielded 13 bands, among which 66,40,20.5 and 14KD were major antigens with the finding of charateristic 71,69,68 and 20.5KD bands and few bands between 59 and 24KD.These results allow us to establish two protein patterns for E.granulosus isolates:ovine bovine and human.