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响应曲面法提取柳杉总黄酮的工艺研究
引用本文:牟新利,吴汉夔,付川,张丽莹,文畅,欧容.响应曲面法提取柳杉总黄酮的工艺研究[J].贵州农业科学,2009,37(10).
作者姓名:牟新利  吴汉夔  付川  张丽莹  文畅  欧容
作者单位:1. 重庆三峡学院化学与环境工程学院,重庆,万州,4040001
2. 南京大学医药生物技术国家重点实验室,江苏,南京,210093
基金项目:重庆三峡学院青年基金研究成果"三峡库区杉科植物药用活性成分研究" 
摘    要:为了研究提取柳杉总黄酮的提取工艺条件,采用响应曲面法,对影响因素的浓度、温度、固液比、时间及乙醇浓度进行了评价.试验得出,最优提取条件为温度76℃,固液比1:31,提取时间3.2h,乙醇浓度76%.在此条件下,柳杉总黄酮的产率为2.163%,响应曲面法能够较好地提取柳杉总黄酮.

关 键 词:柳杉  提取  响应曲面法  总黄酮

Study on Extraction Technology of Flavonoids in Cryptomeria fortunei by Response Surface Methodology
MOU Xin-li,WU Han-kui,FU Chuan,ZHANG Li-ying,WEN Chang,OU Rong.Study on Extraction Technology of Flavonoids in Cryptomeria fortunei by Response Surface Methodology[J].Guizhou Agricultural Sciences,2009,37(10).
Authors:MOU Xin-li  WU Han-kui  FU Chuan  ZHANG Li-ying  WEN Chang  OU Rong
Abstract:The concentration, temperature, solid/liquid ratio, time and alcohol concentration affecting extraction rate of flavonoids in Cryptomeria fortunei were evaluated by using response surface methodology to study the optimum extraction technology. The results showed that the 2.163% flavonoids extraction rate could be obtained under the optimum extraction conditions of 76℃, 1 : 31 of solid/liquid ratio, 3.2 h of extraction time and 76% alcohol concentration and the response surface methodology can be used in flavonoids extraction in Cryptomeria fortunei.
Keywords:Cryptomeria fortunei  extraction  response surface methodology  flavonoids
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