光周期对野生卷丹试管苗鳞茎形成及糖代谢的影响

为了探索光周期对百合试管鳞茎生长发育的诱导效应，寻求试管鳞茎生产的最佳光照参数，以秦巴山区野生卷丹（Lilium lancifolium）试管苗为材料，采用5种不同光周期处理（每天分别暗处理0、8、12、16和24 h），对试管小鳞茎的发生和膨大、糖含量、淀粉酶活性及其对培养基糖源利用情况进行了测定。研究结果表明：不同光周期处理对试管小苗的形态建成和生长发育影响显著，以16 h暗处理对小鳞茎的发生和膨大最为有利；不同培养时期测定表明，经不同光周期处理的小鳞茎可溶性总糖含量、淀粉含量及淀粉酶活性等呈现一定差异；经60 d培养，短光照和黑暗处理（黑暗12、16和24 h）的小鳞茎淀粉含量比全光照和长光照（0和8 h）处理的高；不同光周期处理下试管鳞茎对培养基蔗糖利用率不同，以短光照（黑暗16 h · d-1）处理的利用效率最高。

The Effects of the Photoperiods on the Bulblet Formation and Sugar Metabolism Change of Wild Lilium lancifolium in Vitro

In order to get the optimum photoperiod parameter for the production of bulblet by tissue culture，the experiment was conducted with 5 photoperiod treatments (darkness time per day：0，8，12，16，24 h) to investigate the effect of photoperiods on the bulblet morphogenesis，amylase activity，as well as the utilization rate of sucrose in the culture medium absorbed by bulblet of the wild Lilium lancifolium Thunb. in vitro. The results showed that the different periods of darkness had a significant effect on the organ formation (root，leaf and bulblet) and the weight of bulblets in test tube，the treatment 16 h · d-1 darkness had better effect for the bulblet formation and enlargement. There were differences between total soluble sugar content，starch content and the amylase activity in bulblets with different photoperiod treatments. After 60 days culture in the test tube，the starch content in bulblets with treatments of 12，16，24 h · d-1 darkness was higher than that with 0，8 h · d-1. The utilization rate of sucrose in the culture medium for bublets was also different among all treatments；The 16 h · d-1 darkness was optimum photoperiodtreatment for bulblet production with tissue culture technology.