Continuous in vitro propagation of Cowdria ruminantium (Welgevonden stock) in a canine macrophage-monocyte cell line |
| |
Authors: | Zweygarth E Josemans A I |
| |
Affiliation: | Onderstepoort Veterinary Institute, Parasitology Division, South Africa. |
| |
Abstract: | The Welgevonden stock of Cowdria ruminantium, aetiologic agent of heartwater, was continuously propagated in DH82 cells, a continuous canine macrophage-monocyte cell line. Cultures of DH82 cells were readily infected provided that the culture medium was supplemented with cycloheximide. Cultures were split at regular 3-day intervals and infection rates ranged between 60% and 95%. Cultures were continuously propagated through more than 125 passages over a period of more than one year. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|