| 不同剂量PMSG和hCG对小鼠超排效果的影响研究 |
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| 引用本文： | 高善颂,李树明. 不同剂量PMSG和hCG对小鼠超排效果的影响研究[J]. 浙江畜牧兽医, 2006, 31(1): 6-8 |
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| 作者姓名： | 高善颂 李树明 |
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| 作者单位： | 重庆市牧草与草食家畜重点实验室,重庆,北碚,400716 |
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| 摘 要： | 选用6周龄雌小鼠48只，随机分为4组，分别用2．5IU、5．0IU、7．5IU、10．0IU的PMSG注射，48h后分别注射hCG2．5IU、5．0IU、7．5IU、10．0IU：次日早晨检栓，60h后冲胚。总见栓率为81．25％（39／48）。冲胚结果：2．5IU、5．0IU、7．5IU和10．0IU剂量组平均每只获胚分别为9．17（110／12）枚、32．67（392／12）枚、16．00（92／12）枚、11．67（140／12）枚，各组超排效果差异极显著（P〈0．01）。结果表明：采用5．0IU PMSG＋5．0IU hCG对小鼠超排效果最好，输卵管分段冲胚法可行性良好。
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| 关 键 词： | 超排 冲胚方法 小鼠 |
| 文章编号： | 1005-7307（2006）01-0006-03 |
| 收稿时间： | 2005-10-11 |
| 修稿时间： | 2005-10-11 |
Superovulation of Mouse Influenced by Various Dose of PMSG and hCG |
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| Gao Shan-song,Li Shu-ming. Superovulation of Mouse Influenced by Various Dose of PMSG and hCG[J]. Zhejiang Journal of Animal Science and Veterinary Medicine, 2006, 31(1): 6-8 |
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| Authors: | Gao Shan-song Li Shu-ming |
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| Abstract: | Divide 48 mice into 4 groups,then respectively inject PMSG 2.50IU,5.0IU,7.5IU and 10.0IU.48 hours later,inject hCG 2.5IU,5.0IU,7.5IU and 10.0IU respectively,then combine cages;check embolus the next morning,and the rate of seeing embolus is 81.25%(39/48).60 hours later wash embryos out,and the result is that:(1)average 9.17(110/12)embryos per mouse on the condition of 2.5IU PMSG+2.5IU hCG;(2)average 32.67(392/12)embryos per mouse on the condition of 5.0IU PMSG+5.0IU hCG;(3)average 16.00(192/12)embryos per mouse on the condition of 7.5IU PMSG+7.5IU hCG;(4)average 11.67(140/12)embrys per mouse on the condition of 10.0IU PMSG+10.0IU hCG.The conclusion is that the 5.0IU PMSG+5.0IU hCG is the best dose and that collecting embryos in virtue of segmenting the oviduct is and effectual way. |
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| Keywords: | PMSG hCG |
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