Identification of<Emphasis Type="Italic">Trichoderma</Emphasis> biocontrol isolates to clades according to ap-PCR and ITS sequence analyses |
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| Authors: | M?Maymon D?Minz O?Barbul A?Zveibil Y?Elad Email author" target="_blank">S?FreemanEmail author |
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| Institution: | (1) Dept. of Plant Pathology, ARO, The Volcani Center, 50250 Bet Dagan, Israel;(2) Inst. of Soil, Water and Environmental Sciences, ARO, The Volcani Center, 50250 Bet Dagan, Israel |
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| Abstract: | A collection ofTrichoderma isolates, with different biocontrol capabilities, were identified by molecular methods. Arbitrarily-primed PCR (ap-PCR) using
repeat motif primers was performed on DNA from aTrichoderma spp. collection of 76 isolates, and representative isolates were further characterized into three main clades by internal
transcribed spacer (ITS) sequence analysis. Consequently, a reliable phylogenetic tree was constructed containing isolates
belonging to theT. harzianum clade (comprisingT. aureoviride, T. inhamatum, andT. virens), theT. longibrachiatum andT. saturnisporum cluster, and that including the speciesT. asperellum, T. atroviride, T. koningii andT. viride.
http://www.phytoparasitica.org posting July 14, 2004. |
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| Keywords: | Anthracnose Fragaria × ananassa gray mold internal transcribed spacer (ITS) region arbitrarily-primed polymerase chain reaction (ap-PCR) ribosomal DNA |
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