Fingerprinting in cucumber and melon (<Emphasis Type="Italic">Cucumis</Emphasis> spp.) Genotypes using morphological and ISSR markers |
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Authors: | Rajiv Krishna Parvathaneni Senthil Natesan Ashok Arunachalam Devaraj Raveendran Muthuraja Rajasree Venkatachalam Arun Prathap Subramani Pugalendhi Laxmanan |
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Institution: | 1.Department of Plant Molecular Biology and Biotechnology, Centre for Plant Molecular Biology,Taminadu Agricultural University,Coimbatore,India;2.Vegetable Research Station,Palur,India;3.Department of Vegetable Crops, Horticultural College and Research Institute,Taminadu Agricultural University,Coimbatore,India |
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Abstract: | In the present investigation, 13 Cucumis genotypes from different geographical areas of India were screened for genetic diversity using 19 morphological traits and
15 ISSR primers. The analysis of morphological traits grouped the accessions into six clusters. Cluster V contained the maximum
number of genotypes namely Kanivellari, Long Green, Andaman Local, Perundurai Local, and Sempatti Local. Clusters I and VI
contained the minimum number of genotypes. Among all the characters, the highest mean value was observed in fruit length (23.38)
and the lowest mean value was observed in stripes on the blossom end (1.31). The 15 ISSR primers generated 109 polymorphic
alleles. The average number of ISSR alleles generated was 8.3 per primer and the level of polymorphism was 87.20%. The ISSR
primer UBC 825 was highly informative with a PIC value of 0.8934. The 13 genotypes were grouped into six clusters based on
ISSR markers. Cluster III contained the maximum number of genotypes, namely Kanivellari, Sankagiri Local, Perundurai Local,
Long Melon, and Sempatti Local, while Clusters I, II, IV, and V (Karur Local, Andaman Local, Edapaddi Local, and N 78, respectively)
contained the minimum number of genotypes. The ISSR profile generated genotypes specific allele namely, UBC 812700bp and UBC 8121000bp for Cluster VI which contained Cucumis genotypes collected from the northern part of India. Similarly, UBC 808 produced specific allele UBC 808650bp formed in Cluster III which contained genotypes collected from Tamil Nadu and Kerala. |
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