水仙温度诱导脂质运载蛋白基因NtTIL的克隆与表达分析

采用PCR法，从水仙（Narcissus tazetta var. chinensis）鳞茎主芽中分离克隆到1个温度诱导脂质运载蛋白基因NtTIL。该基因含有567 bp开放阅读框，编码188个氨基酸。该基因属于lipocalin-2 superfamily基因家族；推导的氨基酸序列含有SCR1、SCR2和SCR3 3个植物lipocalin的典型结构域，与小麦、拟南芥等植物的同源性大于75%；编码的蛋白为稳定酸性亲水蛋白，不含有信号肽，进行N末端朝外由外到内的跨膜运动。qRT-PCR结果分析表明，水仙生长发育过程中鳞茎膨大期NtTIL表达量较低；休眠期表达量先升高后降低。推测NtTIL对水仙鳞茎主芽休眠具有一定调控作用。

Cloning and Expression Analysis of Temperature-induced Lipocalin in Narcissus tazetta var.chinensis

An encoding temperature-induced lipocalin（TIL）gene NtTIL was separated and cloning by PCR in Narcissus tazetta var. chinensis. The open reading frame of NtTIL was 567 bp，encoding a protein of 188 amino acids. Biological software for NtTIL protein analysis showed that：The gene belongs to lipocalin-2 superfamily gene family；typical domains deduced from amino acid sequence contained SCR1，SCR2 and SCR3 three of botanical lipocalin. It’s homology greater than wheat，Arabidopsis and other plants 75%. Sequence analyses revealed that this protein was stable and hydrophilic without signal peptides；transmembrane movement was from outside to inside in the outward N-terminus，then transmembrane. And the relative expression of this gene was surveyed by real-time quantitative PCR during different growth stage. The results showed that its relative expression during different growth stage was different；the NtTIL expression was low in bulb swelling stage，expression increased and then reduced in dormancy stage，and expression increased slightly in flower differentiation stage. In conclusion，itsuggested that NtTIL could play a role for regulation of bud dormancy in Narcissus tazetta var. chinensis.