| 家蚕脂肪体原代培养中各种形态细胞的观察 |
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| 引用本文： | 刘敏,潘敏慧,吴克军,鲁成.家蚕脂肪体原代培养中各种形态细胞的观察[J].西南农业学报,2010,23(1). |
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| 作者姓名： | 刘敏 潘敏慧 吴克军 鲁成 |
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| 作者单位： | 1. 云南省农业科学院蚕桑蜜蜂研究所,云南,蒙自,661101
2. 西南大学蚕学与系统生物学研究所,农业部蚕桑学重点开放实验室,重庆,400716 |
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| 基金项目： | 国家重点基础研究发展计划"973"项目，国家高技术研究计划"863"项目，国家自然科学基金项目 |
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| 摘 要： | 取家蚕4~5龄幼虫背部脂肪体,采用胰蛋白酶消化和组织块贴壁法进行离体培养,观察脂肪体细胞在体外培养过程中的变化。结果表明,家蚕脂肪体细胞在原代培养过程中出现了一批具有各种形态的脂肪前体细胞,细胞间呈管道状或网状排列等,可在体外培养条件下繁殖。最后这些细胞充满油滴后陆续死亡。该观察结果可为进一步探讨家蚕脂肪体细胞的分化及脂类代谢调节机制提供实验条件。
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| 关 键 词： | 家蚕 脂肪体细胞 原代培养 |
Observation of Different Shape Cells on Primary Culture of Fat Body Cells from Bombyx mori |
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| LIU Min,PAN Min-hui,WU Ke-jun,LU Cheng.Observation of Different Shape Cells on Primary Culture of Fat Body Cells from Bombyx mori[J].Southwest China Journal of Agricultural Sciences,2010,23(1). |
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| Authors: | LIU Min PAN Min-hui WU Ke-jun LU Cheng |
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| Abstract: | The dorsal fat bodies of silkworm larva in 4th and 5th instars were obtained and cultured in vitro with trypsin-digest method and tissue pieces-stick method.During the course of differentiation of precursor lipocytes from silkworm,several shape cells were observed.They were pipeline-like or reticulately arranged and could grow. Finally the lipocytes ruptured to death after secreting a great deal of lipid droplets.The results would provide the experimental condition for study of differentiation of fat body cells and mechanism of lipid metabolism of Bombyx mori. |
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| Keywords: | Bombyx mori Fat body cell Primary culture |
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