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| 山东省猪瘟流行毒株E2基因主要抗原编码区序列差异分析 | |
| 引用本文： | 叶丙鑫,兰邹然,田夫林,单虎,曾巧英.山东省猪瘟流行毒株E2基因主要抗原编码区序列差异分析[J].甘肃农业大学学报,2010,45(1). |
| 作者姓名： | 叶丙鑫 兰邹然 田夫林 单虎 曾巧英 |
| 作者单位： | 1. 甘肃农业大学动物医学院,甘肃,兰州,730070 2. 山东省动物疫病预防与控制中心,山东,济南,250022 3. 青岛农业大学,山东,青岛,266109 |
| 基金项目： | 国家自然科学基金(30571389);;甘肃省农业生物技术研究与开发项目(GNSW-2008-09);;公益性行业(农业)科研专项(200803020) |
| 摘 要： | 用RT-PCR方法对山东省猪瘟病毒(CSFV)流行毒株的E2基因进行克隆,用DNAstar软件对获得的CSFV及已发表的CSFV毒株E2基因核苷酸和氨基酸序列进行了比较和分析,构建了CSFV遗传发生树.结果表明:扩增的12株猪瘟流行病毒的E2基因长度均为270bp,与预期大小一致;12株病毒株均属于1个组群中的2个亚群,其中,病毒株SDTA-1、SDTA-2属于同一个亚群,E2基因与疫苗株HCLV相比,核苷酸、氨基酸同源性分别为83.5%、88.9%,其余10株病毒株属于同一个亚群,E2基因与疫苗株HCLV相比,核苷酸、氨基酸同源性分别为80.5%～83.5%、82.2%～84.4%. |
| 关 键 词： | 猪瘟 RT-PCR 流行毒株 E2基因 序列分析 进化树 |
Cloning and sequence analysis of E2 gene of twelve CSFV strains in Shandong |
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| YE Bing-xin,LAN Zou-ran,TIAN Fu-lin,SHAN Hu,ZENG Qiao-ying.Cloning and sequence analysis of E2 gene of twelve CSFV strains in Shandong[J].Journal of Gansu Agricultural University,2010,45(1). | |
| Authors: | YE Bing-xin LAN Zou-ran TIAN Fu-lin SHAN Hu ZENG Qiao-ying |
| Institution: | 1.College of Veterinary Medicine;Gansu Agricultural University;Lanzhou 730070;China;2.Animal Disease Prevention and Control Center of Shandong Province;Jinan 250022;3.Qingdao Agricultural University;Qingdao 266109;China |
| Abstract: | The major antigen coding region sequences of E2 gene of twelve recent prevalent field classical swine fever viruses(CSFV)collected from Shandong province were obtained with reverse trancriptase polymerse chain reaction(RT-PCR)and sequencing.The abovementioned sequences were analysed with DNAstar and compared with the previously published sequences of references strains.The phylogenetic tree was constructed.The results showed that all the twelve prevalent CSFV strains belonged to gene group 2 which was divid... |
| Keywords: | classical swine fever RT-PCR prevalent strains E2gene sequence analysis phylogenetic tree |
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