| 铁皮石斛的组织培养与快速繁殖研究 |
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| 引用本文： | 周俊辉,钟雪锋,蔡丁稳.铁皮石斛的组织培养与快速繁殖研究[J].仲恺农业技术学院学报,2005,18(1):23-26. |
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| 作者姓名： | 周俊辉 钟雪锋 蔡丁稳 |
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| 作者单位： | 仲恺农业技术学院园艺园林系,广东,广州,510225 |
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| 摘 要： | 以铁皮石斛(Demdrobium cardidum Wall.ex Lindl)无菌幼苗茎段为外植体,分别研究了不同基本培养基、天然附加物和植物生长调节剂对铁皮石斛原球茎、不定芽诱导、增殖和生根的影响.结果表明,在不定芽诱导和增殖中,4种基本培养基中以B5的效果最好.而芽增殖率则以B5基本培养基中添加O.1 mg/L6-BA的效果最好;在3种天然附加物中,以添加椰子汁对芽的增殖效果最好;原球茎的增殖以1.0 mg/L的NAA效果最佳,诱导生根则以1.0 mg/L的NAA效果最明显.
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| 关 键 词： | 铁皮石斛(Demdrobiurm cardidum Wall.ex Lndl) 组织培养 快速繁殖 |
| 文章编号： | 1006-0774(2005)01-0023-04 |
| 修稿时间： | 2004年11月28 |
Study of tissue culture and rapid propagation in Dendrobium candidum |
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| ZHOU Jun-hui,ZHONG Xue-feng,CAI Ding-wen.Study of tissue culture and rapid propagation in Dendrobium candidum[J].Journal of Zhongkai Agrotechnical College,2005,18(1):23-26. |
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| Authors: | ZHOU Jun-hui ZHONG Xue-feng CAI Ding-wen |
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| Abstract: | The effects of various basic media, nature mash, 6-BA and NAA on the stem culture of Dendrobium candidumwere studied. Among 4 media, the B5 medium was the best for bud inducing and proliferating; the highest efficiency of bud proliferation was obtained by the use of B5 medium with the addition of 0.1 mg/L 6-BA, coconut milk; the best effect of protocorm proliferation was obtained by the use of B5 medium with the addition of 1.0 mg/L NAA; the best effect of root inducing was obtained by the use of B5 medium with the addition of 1.0 mg/L NAA. |
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| Keywords: | Dendrobium candidum tissue culture rapid propagation |
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