板栗品种资源分子水平遗传多样性研究

板栗为中国栗属的代表种,广泛分布于中国的寒温带、温带和暖温带,其分布区地形复杂,由于条件各异,形成了极其丰富的板栗品种。采用AFLP技术对收集到的86个板栗品种进行了分子水平的遗传多样性研究,结果表明,供试板栗群体间的遗传多样性(Dst)为0.072 8,群体内遗传多样性(Hs)为0.080 4,群体分化系数Gst为0.475 3,基因流Nm为0.452 9,供试板栗群体内分化比较严重。通过对AFLP分析的各个步骤进行反复试验,证明了以AFLP-荧光法来分析板栗品种资源分子水平遗传多样性是可行的方法。

Study on Heritance Diversity of Chinese Chestnut ( Castanea mollissima ) Variety Resources at Molecular Level

As the representative Species of China castanea,Chinese chestnut widely ranged to Chinese cool temperature zone,temperate zone and mild temperate zone,whose areal landform is intricacy,considering qualification each strange,most abundance multiple Chinese chestnut Bred were formed.It was urgent to find a highly active and fleet(identification) of variety analytic numerator labelling technique.Inheritance diversity of 86 Chinese chestnut varieties were researched in the article at molecular level with AFLP technique,the results showed that: the inheritance diversity(dst)of Chinese chestnut colony in the test was 0.072 8,colony inside inheritance diversity(hs)was 0.080 4,colony coefficient of differentiation Gst was 0.475 3,gene flow Nm was 0.452 9,the colony internal division of Chinese chestnut for test was relatively serious.Through the medium of versus AFLP analytic each step proceed trial and error,it was witnessed that AFLP-fluorescence method was a feasible analytical procedure to clarify inheritance diversity at molecular level of Chinese chestnut quality resource.