| 仔猪黄痢K88-K99-987p-F41多价菌毛疫苗的制备及小鼠免疫试验 |
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| 引用本文： | 李鹏,王家乡,程碧军. 仔猪黄痢K88-K99-987p-F41多价菌毛疫苗的制备及小鼠免疫试验[J]. 动物医学进展, 2006, 27(9): 55-58,96 |
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| 作者姓名： | 李鹏 王家乡 程碧军 |
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| 作者单位： | 长江大学动物科学学院,湖北荆州,434025;华中农业大学动物医学院,湖北武汉,430070 |
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| 基金项目： | 湖北省科技厅科技攻关项目 |
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| 摘 要： | 为了更好地预防仔猪黄痢，选取野生分离菌株HN2001（K88ab）、HN2002（K88ac）、HN2003（K88ad）、HN2004（K99）、HN2005（987p）和HN2006（F41）培养后用低温磁力搅拌法提取菌毛，制成5批多价菌毛混合油乳剂灭活苗。试验结果表明，5批多价灭活疫苗对小鼠的平均保护率达95．0％，为进一步进行本体动物试验提供了有价值的参考资料。
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| 关 键 词： | 大肠埃希菌 K88-K99-987p-F41多价菌毛疫苗 研制 |
| 文章编号： | 1007-5038（2006）09-0055-05 |
| 收稿时间： | 2006-05-11 |
| 修稿时间： | 2006-05-11 |
Study on the Preparation of Multivalent Pili Oil-emulsion Vaccine for Yellow Scour of Piglets |
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| LI Peng,WANG Jia-xiang,CHENG Bi-jun. Study on the Preparation of Multivalent Pili Oil-emulsion Vaccine for Yellow Scour of Piglets[J]. Progress In Veterinary Medicine, 2006, 27(9): 55-58,96 |
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| Authors: | LI Peng WANG Jia-xiang CHENG Bi-jun |
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| Affiliation: | 1. College of Animal Science ,Yangtze University, Jingzhou, Hubei, 434025, China ; 2. College of Animal Medicine, Huazhong Agricultural University ,Wuhan , Hubei, 430070, China |
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| Abstract: | In order to prevent yellow scour of the newborn piglets well,the wild isolated strains HN2001(K88ab),HN2002(K88ac),HN2003(K88ad),HN2004(K99),HN2005(987p) and HN2006(F41) were selected as the vaccine strains to cultivate.The pili of the cultivated strains were purified with stirring way by bar magnet in low temperature,and 5 batches of multivalent pili oil-emulsion vaccines were produced.The results of protection experiments in mices showed that 95%(38/40) of mices were protected.The research provide the theory basis for further field test and one kind of biological preparation with more comprehensive extent to prevent and control the newborn pig diarrhea in practice. |
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| Keywords: | E. coli K88-K99-987p-F41 multivalent pili oil-emulsion vaccine mouse imune test |
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