| 基于Cre/loxP重组系统的多基因载体构建及烟草转化研究 |
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| 引用本文： | 贾香楠,李伟,沈俊岭,欧阳昆唏,陈晓阳.基于Cre/loxP重组系统的多基因载体构建及烟草转化研究[J].北京林业大学学报,2010,32(5):121-125. |
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| 作者姓名： | 贾香楠 李伟 沈俊岭 欧阳昆唏 陈晓阳 |
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| 作者单位： | 1 北京林业大学林木育种国家工程实验室 2 华南农业大学林学院 |
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| 基金项目： | 教育部科学技术研究重点项目 |
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| 摘 要： | 利用一套基于Cre/loxP重组系统的植物多基因表达载体系统将苏云杆菌毒蛋白基因(BtCryIAc)、甜菜碱醛脱氢酶基因(BADH)、GA20氧化酶基因(pttGA20ox)和rolB基因构建于植物表达载体pYL1305上,命名为pYL1305BBGR。采用农杆菌介导的叶盘法转化烟草,经PCR和Southern blot检测发现,多基因已成功转入烟草基因组中;经半定量RT-PCR检测证实,4个基因均能正常表达。
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| 关 键 词： | Cre/loxP 重组系统 多基因表达载体 BtCryIAc基因 BADH基因 pttGA20ox基因 rolB基因 |
| 收稿时间： | 1900-01-01 |
Construction of muiti-gene expression vector and tobacco transformation based on Cre-LoxP recombination system |
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| JIA Xiang-nan,LI Wei,SHEN Jun-ling,OUYANG Kun-xi,CHEN Xiao-yang.Construction of muiti-gene expression vector and tobacco transformation based on Cre-LoxP recombination system[J].Journal of Beijing Forestry University,2010,32(5):121-125. |
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| Authors: | JIA Xiang-nan LI Wei SHEN Jun-ling OUYANG Kun-xi CHEN Xiao-yang |
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| Institution: | 1 National Engineering Laboratory of Forest Tree Breeding，Beijing Forestry University，100083，P.R.China; 2 College of Forestry, South China Agricultural University, Guangzhou，510642，P.R.China. |
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| Abstract: | Using a system of multi-gene plant expression vector based on a set of Cre-LoxP recombination system, the BtCryIAc, BADH, pttGA20ox and rolB genes were inserted into the plant expression vector pYL1305, which was named pYL1305BBGR. The tabacco was transformed by leaf disc method mediated by Agrobacterium tumefaciens. Tested by PCR and Southern blot, the multi genes have been successfully transferred into the genome of tobacco. The semi-quantitative RT-PCR analysis showed that all the 4 genes were expressed normally. |
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| Keywords: | Cre-loxP recombination system multi-gene expression vector BtCryIAc gene BADH gene pttGA20ox gene rolB gene |
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