| 鹅细小病毒QY分离株NS2基因的克隆及序列分析 |
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| 引用本文： | 李福伟,李惠敏,张桂红,曹顶国.鹅细小病毒QY分离株NS2基因的克隆及序列分析[J].水禽世界,2007(8):6-8. |
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| 作者姓名： | 李福伟 李惠敏 张桂红 曹顶国 |
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| 作者单位： | 1. 山东省农业科学院家禽研究所,济南,250023
2. 华南农业大学兽医学院,广州,510642 |
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| 摘 要： | 参照GenBank中收录的鹅细小病毒(GPV)B株基因序列设计并合成了扩增广东清远分离株(QY株)NS2基因的一对引物,利用PCR技术扩增出来长约1.5kb的目的片断,将其克隆到PMD18-T载体上,筛选重组质粒并进行了序列测定及分析,测序结果表明,GPV分离株NS2基因由1356个核苷酸组成,编码451个氨基酸。经与B株、DN株、FS株进行同源性比较,核苷酸的同源性分别为99.1%、99%和99%;推导的氨基酸同源性分别为98.7%、98.7%和987%。
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| 关 键 词： | 鹅细小病毒 NS2基因 克隆 序列分析 |
| 文章编号： | 1673-1085（2007）08-0006-03 |
| 收稿时间： | 2007-07-06 |
| 修稿时间： | 2007年7月6日 |
Cloning and sequencing of NS2 genes of goose parvovirus QY strain |
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| LI Fu-wei,LI Hui-min,ZHANG Gui-hong,CAO Ding-guo.Cloning and sequencing of NS2 genes of goose parvovirus QY strain[J].Poultry Science,2007(8):6-8. |
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| Authors: | LI Fu-wei LI Hui-min ZHANG Gui-hong CAO Ding-guo |
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| Institution: | 1.Institute of poultry Shandong Academy of Agricultural Sciences ,jinan shandong 250023, China; 2 College of Veterinary Medicine ,South China Agricultural University, Guangzhou 510642, China |
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| Abstract: | According to the published genome sequence of goose parvovirus B strain ,oen pair of primer was designed to amplify NS2 gene from goose parvovirus Qingyuan(QY) strain(from Guangdong Province)by PCR. 1.5kb segment was amplified. The PCR products were cloned into pM D 18-T vector.Identified and sequenced ,Nucleotide sequence of NS2 genes of the QY strain was 1356bp in length ,encoding 451 amino acids .In comparison with the B Strain ,DS strain and FS strain ,the nucleotide homology were 99.1%,99% and 99% respectively ,and the nucleotide homology of NS2 gene were 98.7%,98.7% and 98.7% respectively. |
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| Keywords: | goose parvovirus NS2gene clone sequence |
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