四川小麦主栽品种酯酶同工酶遗传差异分析

对四川近５０ 年来年推广面积６－６７ 万ｈｍ２（１００ 万亩） 以上的４０ 个主栽小麦品种进行了酯酶同工酶聚丙烯酰胺凝胶电泳分析。结果表明，１６ 条酯酶同工酶酶带中有７ 条酶带呈现多态性，其余９ 条酶带在所有供试品种中无差异，为四川栽培小麦品种的基本带。４０ 个小麦品种具有７ 种酯酶酶谱，其中３２ 个品种具有完全相同的酶谱，该酶谱为四川主栽小麦品种的优势酶谱。另外，８ 个品种具有６ 种酶谱类型，表明这些品种在同工酶水平上的分子特异性，可以作为这些品种鉴定的同工酶指纹。

The Study on Genetic Variation of Esterase Isozymes of Sichuan Major Wheat Cultivars

In this study, the esterase isozymes were analysed with polyacrylamide gel electrophoresis in the 40 wheat cultivars, which has once popularized above 6 67×10 4hm 2 per year in Sichuan during the last 50 years.The results shows as follows: 16 bands are seperated by electrophoresis and 7 of them were polymorphism.All of the studied cultivars has 9 identical bands, which are the common bands in Sichuan wheat cultivars. 7 different zymograms are observed in the 40 cultivars, among which type-1 distributed in 32 cultivars. So type-1 is the preponderant zymogram. The remainder 8 cultivars have 6 zymograms. Based on this result, it is concluded that esterase zymograms could be used as the fingerprint to iderntify some cultivars.