细胞分裂素对蝴蝶兰体细胞胚诱导及大分子代谢研究

以含6 BA、2,4 D培养基诱导蝴蝶兰外植体。6 BA可从嫩茎切块诱导出胚性愈伤组织,细胞富含淀粉粒,而2,4 D或2,4 D加6 BA诱导得到的愈伤组织不能分化。6 BA易于从嫩叶切片诱导胚性愈伤组织,进行体细胞胚发生过程的显微观察。在6 BA诱导嫩叶切片外植体期间,DNA含量前期平缓上升,第25d后急剧上升。RNA含量开始诱导就上升,第11~18 d达到高峰。水溶性蛋白含量上升启动稍晚,在第25 d达到高峰。

Effect of cytokinin on the induction and macromolecules metabolic kinetics of somatic embryogenesis in Phalaenopsis sp.

Two ex-plants from test tube seedling of Phalaenopsis sp.were cultured on the media containing 6-BA and 2,4-D.From the stem ex-plant,embryogenic callus(EC)with large amounts of starch granules in cell could be induced with the presence of 6-BA.On the other hand,the callus induced with 2,4-D or with both 2,4-D and 6-BA were unable to differentiate.EC could also be induced efficiently from the leaf ex-plant cultured on the medium containing 6-BA.Histological observation and metabolic analysis conducted during somatic embryogenesis showed that,in leaf ex-plant,the DNA content increased slowly initially and sharply on the 25th day of induction.The RNA content increased when induction began,and reached a peak on the 11-18th day.The soluble protein content started to change later than RNA and peaked on the 25th day.