| 柑橘类果树组织总RNA优异且通用的提取方法 |
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| 引用本文： | 叶庆亮,彭爱红,曹立,江东,钟广炎.柑橘类果树组织总RNA优异且通用的提取方法[J].江西农业大学学报,2007,29(5):732-735,739. |
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| 作者姓名： | 叶庆亮 彭爱红 曹立 江东 钟广炎 |
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| 作者单位： | 1. 西南大学,园林园艺学院,重庆,400716;中国农业科学院,柑橘研究所,重庆,400712
2. 中国农业科学院,柑橘研究所,重庆,400712 |
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| 基金项目： | 科技部“973”重大基础研究前期研究专项(2005CCA02900)，重庆市自然科学基金(SCTC2005BA1010) |
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| 摘 要： | 分别以柚类、柠檬、甜橙、枳壳和宽皮橘的果皮、果肉、枝皮、老叶、嫩叶、树根、子房、花瓣,尤其是以果胶含量丰富的岩溪晚芦果皮等作试材,将两次LiCl沉淀法加以改良,加入适量CaCl2,找到一种柑橘类果树总RNA优异且通用的提取方法,所得RNA的质量和产量均较高。经差减文库构建、连接、电转化,成功获得了目标片段。
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| 关 键 词： | 柑橘 RNA提取 CaCl2处理 果胶 减法文库 |
| 文章编号： | 1000-2286（2007）05-0732-04 |
| 收稿时间： | 2007-05-21 |
| 修稿时间： | 2007-05-212007-08-31 |
An Effective and General Protocol for the Isolation of Total RNA From the Tissues of Citrus Trees |
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| YE Qing-hong,PENG Ai-hong,CAO Li,JIANG Dong,ZHONG Guang-yan.An Effective and General Protocol for the Isolation of Total RNA From the Tissues of Citrus Trees[J].Acta Agriculturae Universitis Jiangxiensis,2007,29(5):732-735,739. |
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| Authors: | YE Qing-hong PENG Ai-hong CAO Li JIANG Dong ZHONG Guang-yan |
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| Institution: | 1.CoUege of Horticulture and Landscape Designing,Southwest University,Chongqing 400712, China;2.Citrus Research Institute, Chinese Academy of Agricultural Sciences, Chongqing 400712,China |
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| Abstract: | Many samples,such as pulp,epicarp,tender leaves,old leaves,ovary,petal et al were tested respectively,especially the epicarp of Yanxiwanlu fruit very rich in polysacchrides.An effective and general protocol for the isolation of total RNA from the tissues of citrus trees was worked out.The protocol,based on two lithium chloride(LiCl) precipitations,is approved by some steps and added with appropriate CaCl2 during RNA extraction.The results Indicated that the RNA is of high quality,as confirmed by isolation of mRNA and subtraction construction of cDNA libraries. |
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| Keywords: | Citrus RNA extraction CaCl2 treatment pectate subtraction library |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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