| 石斛粗多糖脱蛋白方法的研究 |
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| 引用本文： | 罗傲雪,范益军,罗傲霜,何涛,刘静,淳泽.石斛粗多糖脱蛋白方法的研究[J].安徽农业科学,2008,36(29). |
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| 作者姓名： | 罗傲雪 范益军 罗傲霜 何涛 刘静 淳泽 |
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| 作者单位： | 1. 四川农业大学都江堰分校,四川都江堰,611830
2. 中国科学院成都生物研究所,四川成都,610041 |
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| 基金项目： | 四川农业大学都江堰分校科技基金
，
四川省科技支撑计划
，
四川省公益性研究计划
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| 摘 要： | 目的]研究石斛多糖中蛋白质的有效去除方法。方法]以蛋白脱除率和多糖损失率作为检测指标,考察3种不同方法对石斛粗多糖液去除蛋白质的效果。结果]三氯乙酸法脱蛋白效果最好,且其多糖损失率最低。结论]该方法简单、易行,适合于石斛粗多糖中蛋白质的去除。
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| 关 键 词： | 石斛 多糖 硫酸-苯酚法 考马斯亮蓝 |
Study on the Protein Removal of the Crude Polysaccharide from Dendrobium |
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| Abstract: | Objective] The methods for the effective protein removal of the crude polysaccharide from dendrobium were studied.Method] Deproteinization rate and polysaccharide loss rate were used as the detecting index.Effects of three different methods on the protein removal by crude polysaccharide from dendrobium were tested.Result] Trichloroacetic acid showed higher removal percentage of protein and lower loss rate of polysaccharide.Conclusion] The method was simple and easy to apply,and was suitable for the protein removal in crude polysaccharide from dendrobium. |
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| Keywords: | Dendrobium Polysaccharide Phenol-sulfuric acid method Coomassie brilliant blue |
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