不同产地金枣柿遗传变异研究

以浙江省内37份金枣柿和3份农家柿品种为材料,利用SSR分子标记技术,结合表型性状和果实营养成分指标,分析不同产地金枣柿遗传多样性。结果表明:金枣柿枝、叶、芽等表型性状具有丰富的形态多样性,14个表型性状的变异系数为7.23%23.54%,其中萼片长与柿蒂长变异系数最大,果形指数、叶形指数变异系数最小;果实营养成分在单株间的变异丰富,变异系数为9.58%66.21%,平均变异系数为37.89%,变异程度高于表型性状;21对SSR多态性引物可从40份材料中扩增出381个DNA位点,平均多态性位点百分率为92.89%,UPGMA聚类分析显示,所选SSR标记能将供试材料完全区分开,在遗传相似系数0.74时将40个试验单株分为3类,主坐标分析所得结果与聚类分析基本一致。该研究结果为金枣柿种质资源的科学利用提供了依据。

Genetic Variation of Jinzaoshi Persimmon from Different Regions in Zhejiang Province

The germplasm resources of Jinzaoshi persimmon in Zhejiang Province were investigated, so as to probe the genetic diversity of Jinzaoshi persimmon from different regions. The genetic variation of collected germplams, including 37 Jinzaoshi and 3 local varieties, was analyzed by SSR molecular marker, together with phenotypic traits and fruit nutrients contents. The results showed Jinzaoshi persimmon possessed a rich diversity on morphology. The variable coefficient about 14 phenotypic characters ranged from 7.23% to 23.54%. The variable coefficient of sepal length and persimmon calyx length were the largest. The fruit shape index and leaf index had the least variation. The fruit nutrient contents ranged from 9.58% (soluble solid content) to 66.21% (tannin content). Twenty-one primers were used to amplify 381 SSR local in 40 test materials, with an average polymorphism rate of 92.89%. The cluster analysis conducted by UPGMA showed that the materials tested could be distinguished and divided into three major groups at the genetic similarity coefficient of 0.74, which was similar to the relationship of PCoA analysis.