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3D fluorescent in situ hybridization using Arabidopsis leaf cryosections and isolated nuclei |
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| Authors: | Leïla Tirichine Philippe Andrey Eric Biot Yves Maurin Valérie Gaudin |
| Institution: | 1. Laboratoire de Biologie Cellulaire, INRA UR 501, IJPB, Route de Saint-Cyr, F-78026, Versailles, France 6. Institut des Sciences du Végétal, CNRS, avenue de la Terrasse, F-91198, Gif-sur-Yvette, France 2. Neurobiologie de l'Olfaction et de la Prise Alimentaire, INRA UMR 1197, Domaine de Vilvert, F-78350, Jouy-en-Josas, France 3. Université Paris-Sud 11, UMR 1197, F-91400, Orsay, France 4. IFR 144 Neuro-Sud, Paris, France 5. Université Pierre et Marie Curie, Paris, France |
| Abstract: | BackgroundFluorescent hybridization techniques are widely used to study the functional organization of different compartments within the mammalian nucleus. However, few examples of such studies are known in the plant kingdom. Indeed, preservation of nuclei 3D structure, which is required for nuclear organization studies, is difficult to fulfill. |
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