| 磷胁迫下大豆叶片及根系酸性磷酸酯酶酶谱的研究 |
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| 引用本文： | 李志刚,宋书宏,李瑞平,苏辉.磷胁迫下大豆叶片及根系酸性磷酸酯酶酶谱的研究[J].大豆通报,2008(2):21-23. |
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| 作者姓名： | 李志刚 宋书宏 李瑞平 苏辉 |
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| 作者单位： | [1]辽宁省农业科学院,沈阳110161 [2]内蒙古民族大学,通辽028042 |
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| 摘 要： | 通过营养液栽培试验,研究了低磷胁迫下4种不同磷效率基因型大豆叶片和根系的酸性磷酸酯酶(APase)同工酶的酶谱和活性变化.结果表明,大豆叶片和根系APase同工酶含量在低磷处理第10 d、20 d、30 d均比对照高,酶谱染色加深和谱带条数的增加,说明低磷胁迫增加该酶活性,磷高效基因型酶活性升高的幅度大于磷低效基因型.
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| 关 键 词： | 酸性磷酸酯酶酶谱 磷胁迫 大豆 低磷胁迫 大豆叶片 根系 酸性磷酸酯酶 酶谱 研究 stress Phosphorus root leaf soybean phosphatase 磷效率基因型 磷高效 酶活性 谱带 加深 染色 低磷处理 含量 |
| 文章编号： | 1009-2765（2008）02-021-03 |
| 收稿时间： | 2008-01-22 |
| 修稿时间： | 2008年1月22日 |
Acid phosphatase zymogram of soybean leaf and root at Phosphorus stress |
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| Abstract: | The active change and zymogram of acid phosphatase isozyme in leaf and root of 4 soybean genotypes with different phosphorus use efficiency were studied under phosphorus stress in nutrient solution. The content of acid phosphatase isozyme in leaf and root of soybean was higher than that of the control on 10 d,20 d and 30 d after phosphorus stress treatment, while the zymogram dyeing was darker with more band, which showed that this enzymatic activity increased with phosphorus lstress, and the increase in amplitude of enzymatic activity of high phosphorus efficient genotypes was greater than that of low phosphorus efficient genotypes. |
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| Keywords: | Acid phosphatase zymogram Phosphorus stress soybean |
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