鸡痘病毒活疫苗污染禽网状内皮组织 增生症病毒的间接免疫荧光法检测研究

通过向无外源病毒污染的鸡痘病毒活疫苗中添加不同剂量的禽网状内皮组织增生症病毒(REV),然后用间接免疫荧光法(IFA)进行检测,确定了该IFA方法的最低检出量为每500羽份疫苗中污染20 TCID_(50)的REV。使用该方法对国内16家企业生产的60批鸡痘病毒活疫苗进行了检验,结果显示2个企业生产的4批疫苗REV检测为阳性。随机选取5批IFA检测阴性样品和4批IFA检测阳性样品,按鸡检查法进行外源病毒检验,结果两种方法对REV污染的检测结果的符合率为100%。

Research of Detection of Avian Reticuloendotheliosis Virus Contamination in Fowlpox Live Vaccine by Indirect Immunofluorescence Assay

In this study, the fowlpox live vaccine, not contaminated with exogenous viruses, was added with different doses of avian reticuloendotheliosis virus (REV) and REV was detected by indirect immunofluorescence assay (IFA). The results indicated that the lowest detection limit was 20 TCID50 for 500 doses of vaccine. 60 batches of fowlpox live vaccine from 16 companies were selected to detect REV contamination using IFA method. The results showed 4 batches of vaccine were REV positive. 5 batches of REV negative vaccine and 4 batches of REV positive vaccine were selected randomly for exogenous virus detection using chicken test method. The coincidence rate of the two methods was 100%.