| 瓶式平板培养制备稻曲病菌薄壁分生孢子 |
| |
| 引用本文： | 蒲相君,王忠文,张丽丽,张君成.瓶式平板培养制备稻曲病菌薄壁分生孢子[J].植物保护,2018,44(3):130-133. |
| |
| 作者姓名： | 蒲相君 王忠文 张丽丽 张君成 |
| |
| 作者单位： | 广西大学农学院 |
| |
| 基金项目： | 国家现代农业产业技术体系广西创新团队建设(nycytxgxcxtd-04-18-2)；广西自然科学基金(2011GXNSFA018068) |
| |
| 摘 要： | 利用三角瓶装纳马铃薯琼脂培养基制备稻曲病菌薄壁分生孢子。结果发现,瓶装培养基的体积与产孢量无关。各菌株在瓶式培养平板上均能正常形成后代孢子。瓶式培养比常规培养皿平板培养具有更高的产孢效率。产孢平板上产生的孢子收取后,菌落继续培养,新形成的孢子数量大幅下降。由于三角瓶能高效避免污染,因而认为瓶式培养是制备稻曲病菌薄壁分生孢子的理想技术。
|
| 关 键 词： | 稻曲病菌 薄壁分生孢子 产孢 瓶式培养 |
| 收稿时间： | 2017/8/26 0:00:00 |
| 修稿时间： | 2017/9/28 0:00:00 |
Culture method with plate in flask for sporulation of thin-walled conidia of Villosiclava virens |
| |
| PU Xiangjun,WANG Zhongwen,ZHANG Lili,ZHANG Juncheng.Culture method with plate in flask for sporulation of thin-walled conidia of Villosiclava virens[J].Plant Protection,2018,44(3):130-133. |
| |
| Authors: | PU Xiangjun WANG Zhongwen ZHANG Lili ZHANG Juncheng |
| |
| Institution: | College of Agriculture, Guangxi University, Nanning 530004, China |
| |
| Abstract: | A culture method with potato agar plate in flask (flask method) for sporulation of thin-walled conidia of Villosiclava virens was established. The results indicated that no relations existed between conidial yield and medium volume of the potato agar inside the flask. Conidia of new generation were formed normally by the flask method for all isolates examined. Efficiency of sporulation cultured by plate in the flask was higher than that by plate inside a common dish. Conidial yield of new generation was decreased greatly when a plate colony was cultured repeatedly after the conidia were moved out. It was concluded that the flask method was an ideal technique for sporulation culture of thin-walled conidia of V.virens due to the high efficiency of sporulation as well as anti-contamination. |
| |
| Keywords: | Villosiclava virens thin-walled conidia sporulation culture method with plate in flask |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《植物保护》浏览原始摘要信息 |
| 点击此处可从《植物保护》下载免费的PDF全文 |
