| 禾谷镰孢荧光定量检测体系的建立及其在玉米苗枯病上的应用 |
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| 引用本文： | 葛波,杨洋,张申萍,王晓鸣,陈国康,段灿星.禾谷镰孢荧光定量检测体系的建立及其在玉米苗枯病上的应用[J].植物保护学报,2018,45(3):409-415. |
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| 作者姓名： | 葛波 杨洋 张申萍 王晓鸣 陈国康 段灿星 |
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| 作者单位： | 西南大学植物保护学院;中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程 |
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| 基金项目： | 国家重点研发计划（2016YFD0100103），国家现代农业（玉米）产业技术体系（CARS-02） |
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| 摘 要： | 为实现对田间土壤中禾谷镰孢Fusarium graminearum的定量检测,本研究构建了土壤含菌量与玉米苗枯病病情指数的回归模型。基于禾谷镰孢甾醇14α-去甲基化酶基因CYP51C序列,设计特异性引物HQ1-F/HQ1-R,利用引物建立实时荧光定量PCR(RT-q PCR)体系,选取4个玉米自交系品种进行室内苗枯病接种试验,调查其病情指数,利用RT-qPCR体系检测土壤禾谷镰孢含菌量,并对病情指数和土壤禾谷镰孢含菌量进行回归。结果表明,仅禾谷镰孢扩增出目的条带并且可从多种病原菌土壤中检测出。RT-qPCR的熔解曲线具有单一吸收峰,扩增曲线的循环阈值与模板浓度呈良好的线性关系,扩增效率为104.7%,标准曲线为y=-3.2137x+34.9560(R~2=0.9968),最低可检测到1 pg/μL的DNA。随着土壤禾谷镰孢接菌量的增加,单位土壤禾谷镰孢含菌量呈线性增加,即y=13.603x-85.370(R~2=0.9998)。4个玉米品种的病情指数与土壤禾谷镰孢含菌量的回归曲线分别为y=0.0789x+22.0590(R~2=0.7949)、y=0.0304x+7.8686(R~2=0.9579)、y=0.0458x+23.7540(R~2=0.5420)、y=0.0471x+32.0760(R~2=0.6753)。
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| 关 键 词： | 禾谷镰孢 实时荧光定量检测 土壤 玉米苗枯病 预警 |
| 收稿时间： | 2017/8/22 0:00:00 |
Development of fluorescent quantitative detection system for fungal pathogen Fusarium graminearum and its application in maize seedling blight |
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| Ge Bo,Yang Yang,Zhang Shenping,Wang Xiaoming,Chen Guokang and Duan Canxing.Development of fluorescent quantitative detection system for fungal pathogen Fusarium graminearum and its application in maize seedling blight[J].Acta Phytophylacica Sinica,2018,45(3):409-415. |
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| Authors: | Ge Bo Yang Yang Zhang Shenping Wang Xiaoming Chen Guokang and Duan Canxing |
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| Institution: | College of Plant Protection, Southwest University, Chongqing 400715, China;Institute of Crop Sciences/National Key Facility of Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China,College of Plant Protection, Southwest University, Chongqing 400715, China;Institute of Crop Sciences/National Key Facility of Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China,College of Plant Protection, Southwest University, Chongqing 400715, China,Institute of Crop Sciences/National Key Facility of Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China,College of Plant Protection, Southwest University, Chongqing 400715, China and Institute of Crop Sciences/National Key Facility of Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
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| Abstract: | |
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| Keywords: | Fusarium graminearum RT-qPCR soil maize seedling blight early warning |
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