生防菌解淀粉芽胞杆菌Jt84干悬浮剂加工及田间评价

解淀粉芽胞杆菌Jt84菌株对水稻真菌病害具有良好的防治效果。本研究通过喷雾干燥技术,开发一种生防菌新剂型杀菌剂。采用Plackett-Burman试验及Box-Behnken试验,对解淀粉芽胞杆菌Jt84干悬浮剂喷雾干燥工艺进行了优化。结合生产实际确定干悬浮剂制备条件分别为：进风温度118.9℃,进样速率25.7 r/min（约为680 mL/h）,热风流量28.56 M³/h,压力0.2 MPa,出风温度80℃,喷头口径0.7 mm。经喷雾干燥试验验证该理论预测值与实际值无显著差异。田间药效试验表明：水稻破口期喷施解淀粉芽胞杆菌Jt84干悬浮剂75 g/667 m²,对水稻穗颈瘟的防治效果为79.3%,防治效果与三环唑相当,能够有效防治稻瘟病。

Evaluation of Bacillus amyloliquefaciens Jt84 Dry Flowable Formulation Production and Control Effect against Rice Blast in Field

Dry flowable formulations of Bacillus amyloliquefaciens Jt84 with fungicidal activity against the rice blast fungus Magnaporthe oryzae were developed by spray drying. The Plackett-Burman experiments and response surface methodology were applied to optimize the spray drying technology of B. amyloliquefaciens Jt84. According to the evaluation by the response surface model established in this study, the optimum conditions were inlet air temperature of 118.9℃, feed flow rate of 25.7 r/min (680 mL/h), atomization caliber of 0.7 mm, atomization pressure of 0.2 MPa, hot air flow of 28.56 M³/h, and outlet air temperature of 80℃. Field trials showed that control efficacy of B. amyloliquefaciens Jt84 dry flowable formulation at 75 g/667 m² against rice blast was 79.3% at rupturing stage of rice, equal to that of 75% tricyclazole wettable powder.