云南省马铃薯疮痂病致病链霉菌种类组成研究

 为明确云南省马铃薯疮痂病病原菌(Streptomyces spp.)的种类及其生物学特征,自2013年从云南省13个马铃薯主产区采集疮痂病病样,共分离到200株链霉菌,通过温室盆栽致病性试验筛选出67株致病菌。通过形态学、生理生化指标、致病性测定及16S rDNA序列分析对获得的菌株进行鉴定。结果显示,引起云南地区马铃薯疮痂病的病原为10种链霉菌,分别为S. caviscabies、S. anulatus、S. scabies、S. turgidiscabies、S. acidiscabies、S. europaeiscabiei、S. luridiscabiei、S. enissocaesilis、S. griseus和S. aureofaciens。其中S. enissocaesilis和 S. anulatus为优势种群,S. caviscabies、S. anulatus和S. luridiscabiei为国内首次报道的病原菌。因此,认为云南省马铃薯疮痂病菌种类复杂多样。

The pathogenic Streptomyces species causing potato scab in Yunnan Province

The aim of this study is to identify and characterize the species of Streptomyces causing potato scab in Yunnan province. Two hundreds Streptomyces strains were isolated from scab tubers obtained from 13 potato main planting areas in Yunnan Province in 2013, and 67 of them were pathogenic on potato in the greenhouse test. The pathogenic isolates were identified according to pathogenicity test, 16S rDNA sequence analysis, and morphological and biochemical characterization. Results revealed 10 pathogenic Streptomyces species in Yunnan Province, including S. caviscabies, S. anulatus, S. scabies, S. turgidiscabies, S. acidiscabies, S. europaeiscabiei, S. luridiscabiei, S. enissocaesilis, S. griseus and S. aureofaciens. Among them, S. enissocaesilis and S. anulatus are dominant species. S. caviscabies, S. anulatus and S. luridiscabiei are newly discovered pathogens in China. Our study suggests that the Streptomyces species causing potato scab have a rich diversity in Yunnan Province.